Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia)

MATULOVÁ, Petra, Kirsti WITTER and Ivan MÍŠEK. Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia). (Microtus agrestis, Rodentia). Taylor and Francis, 2002, vol. 43, No 43, p. 138–142, 4 pp. ISSN 0300-8207.

Basic information

Original name

Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia)

Name in Czech

Exprese PCNA v zubních základech hraboše mokřadního (Microtus agrestis, Rodentia)

Authors

MATULOVÁ, Petra (203 Czech Republic, guarantor), Kirsti WITTER (276 Germany) and Ivan MÍŠEK (203 Czech Republic)

Edition

(Microtus agrestis, Rodentia), Taylor and Francis, 2002, 0300-8207

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.646

Organization unit

Faculty of Science

Keywords in English

Dentition; Odontogenesis; Proliferation Marker; Embryo; Development

Tags

dentition, development, embryo, Odontogenesis, Proliferation Marker

Tags

International impact, Reviewed

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Abstract

V originále

Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.

In Czech

Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.