MATULOVÁ, Petra, Kirsti WITTER and Ivan MÍŠEK. Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia). (Microtus agrestis, Rodentia). Taylor and Francis, 2002, vol. 43, No 43, p. 138–142, 4 pp. ISSN 0300-8207.
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Basic information
Original name Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole (Microtus agrestis, Rodentia)
Name in Czech Exprese PCNA v zubních základech hraboše mokřadního (Microtus agrestis, Rodentia)
Authors MATULOVÁ, Petra (203 Czech Republic, guarantor), Kirsti WITTER (276 Germany) and Ivan MÍŠEK (203 Czech Republic).
Edition (Microtus agrestis, Rodentia), Taylor and Francis, 2002, 0300-8207.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher France
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.646
Organization unit Faculty of Science
Keywords in English Dentition; Odontogenesis; Proliferation Marker; Embryo; Development
Tags dentition, development, embryo, Odontogenesis, Proliferation Marker
Tags International impact, Reviewed
Changed by Changed by: RNDr. Petra Matulová, CSc., učo 9032. Changed: 18/11/2008 14:07.
Abstract
Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.
Abstract (in Czech)
Cell proliferation in developing tooth germs has been studied particularly using bromodeoxyuridine (BrdU) incorporation into growing tooth primordia and by counting and three-dimensional (3D) reconstruction of mitoses in serial sections of developing teeth. PCNA has been proposed as an alternative marker of proliferation activity. The aim of our study was to detect immunohistochemically locations of PCNA-positive cells in developing tooth germs of Microtus agrestis (Rodentia). PCNA expression could be distinguished in oral epithelium and mesenchyme before first signs of dental lamina elevation. During bud, cap, and bell stages, positive immunostaining could be observed at defined sites in enamel organ, tooth papilla, and dental follicle. Rudimental tooth germs of the upper diastema, enamel knots, and inner enamel epithelium at day of ontogeny 18 and 19 showed negative reaction. PCNAmarks cycling and early G0 cells and can be used successfully as a proliferation marker even in collection material.
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