This book constitutes the refereed Proceedings of the Second CESNET Conference on Security, Middleware, and Virtualization -- Glue of Future Communication Networks held in Prague, Czech Republic, in September 2008. It contains 14 reviewed papers accepted by programme committee. Papers are placed in four thematic parts: Middleware, Security, Network Management and Applications. The book has both Author and Subject index. Suggested reading for researchers interested in actual approaches and research in the area of internet high-speed networking, security and grids.