J 2008

Ribotyping and Whole-Cell Protein Analysis of Spirochetes Isolated from Arthropods in the Czech Republic

BUŇKOVÁ, Leona, Pavel ŠVEC, Jiří HALOUZKA, Ivo RUDOLF, Miroslav NĚMEC et. al.

Basic information

Original name

Ribotyping and Whole-Cell Protein Analysis of Spirochetes Isolated from Arthropods in the Czech Republic

Name in Czech

Ribotypizace a analýzy celkových buněčných proteinů spirochet izolovaných z členovců v České republice

Authors

BUŇKOVÁ, Leona (203 Czech Republic), Pavel ŠVEC (203 Czech Republic), Jiří HALOUZKA (203 Czech Republic), Ivo RUDOLF (203 Czech Republic, guarantor) and Miroslav NĚMEC (203 Czech Republic, belonging to the institution)

Edition

Annals of Agricultural and Environmental Medicine, 2008, 1232-1966

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.443

RIV identification code

RIV/00216224:14310/08:00024347

Organization unit

Faculty of Science

UT WoS

000261767500009

Keywords in English

Borrelia burgdorferi sensu lato; Czech Republic; ribotyping; whole-cell protein analysis; taxonomy

Tags

Tags

International impact, Reviewed
Změněno: 30/7/2013 15:02, Ing. Zdeňka Rašková

Abstract

ORIG CZ

V originále

In the presented work, six Borrelia burgdorferi sensu lato isolates were recovered from Ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from Culex pipiens mosquito collected in the Valtice area (South Moravia, Czech Republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. The ribotype patterns obtained with HindIII and EcoRV ribotype patterns showed good correlations with the recently proposed genotypes and grouped each genospecies in a well-separated cluster. The whole-cell protein profiles of genospecies were more heterogenous than the ribotype patterns. The non-identified spirochetal strain was clearly separated from the Borrelia isolates in both methods thus proving their good capacity to characterize Borrelia burgdorferi sensu lato strains.

In Czech

Práce prezentuje výsledky ribotypizace a analýzy celkových buněčných proteinů šesti kmenů Borrelia burgdorferi sensu lato izolovaných z klíštěte Ixodes ricinus.

Links

GA206/03/0726, research and development project
Name: Ekologie emergentních mikroorganismů přenosných hematofágními členovci
KJB600930613, research and development project
Name: Diverzita kultivovatelných mikroorganizmů v klíšťatech, vektorech patogenů obratlovců
Investor: Academy of Sciences of the Czech Republic, Diversity of cultivable microorganisms of ixodid ticks, recognized vectors of vertebrate pathogens
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time