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@inproceedings{799428,
author = {Maška, Martin and Daněk, Ondřej and OrtizanddeandSolórzano, Carlos and MuñozandBarrutia, Arrate and Kozubek, Michal and Fernández García, Ignacio},
address = {Berlin, Heidelberg},
booktitle = {16th Scandinavian Conference on Image Analysis},
doi = {http://dx.doi.org/10.1007/978-3-642-02230-2_40},
keywords = {nucleus segmentation; touching nuclei; level set framework; contour evolution; topology preservation},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Berlin, Heidelberg},
isbn = {978-3-642-02229-6},
note = {LNCS 5575},
pages = {390-399},
publisher = {Springer-Verlag},
title = {A Two-Phase Segmentation of Cell Nuclei Using Fast Level Set-Like Algorithms},
url = {http://dx.doi.org/10.1007/978-3-642-02230-2_40},
year = {2009}
}
TY - JOUR
ID - 799428
AU - Maška, Martin - Daněk, Ondřej - Ortiz-de-Solórzano, Carlos - Muñoz-Barrutia, Arrate - Kozubek, Michal - Fernández García, Ignacio
PY - 2009
TI - A Two-Phase Segmentation of Cell Nuclei Using Fast Level Set-Like Algorithms
PB - Springer-Verlag
CY - Berlin, Heidelberg
SN - 9783642022296
N1 - LNCS 5575
KW - nucleus segmentation
KW - touching nuclei
KW - level set framework
KW - contour evolution
KW - topology preservation
UR - http://dx.doi.org/10.1007/978-3-642-02230-2_40
N2 - An accurate localization of a cell nucleus boundary is inevitable for any further quantitative analysis of various subnuclear structures within the cell nucleus. In this paper, we present a novel approach to the cell nucleus segmentation in fluorescence microscope images exploiting the level set framework. The proposed method works in two phases. In the first phase, the image foreground is separated from the background using a fast level set-like algorithm by Nilsson and Heyden (2003). A binary mask of isolated cell nuclei as well as their clusters is obtained as a result of the first phase. A fast topology-preserving level set-like algorithm by Maška and Matula (2008) is applied in the second phase to delineate individual cell nuclei within the clusters. The potential of the new method is demonstrated on images of DAPI-stained nuclei of a lung cancer cell line A549 and promyelocytic leukemia cell line HL60.
ER -
MAŠKA, Martin, Ondřej DANĚK, Carlos ORTIZ-DE-SOLÓRZANO, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK a Ignacio FERNÁNDEZ GARCÍA. A Two-Phase Segmentation of Cell Nuclei Using Fast Level Set-Like Algorithms. In \textit{16th Scandinavian Conference on Image Analysis}. Berlin, Heidelberg: Springer-Verlag. s.~390-399. ISBN~978-3-642-02229-6. doi:10.1007/978-3-642-02230-2\_{}40. 2009.
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