Detailed Information on Publication Record
2008
Functionalised magnetic microspheres for molecular diagnostic applications
RITTICH, Bohuslav, Alena ŠPANOVÁ and Daniel HORÁKBasic information
Original name
Functionalised magnetic microspheres for molecular diagnostic applications
Name in Czech
Využití funkcionalizovaných magnetických mikročástic v molekulární diagnostice
Authors
RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic) and Daniel HORÁK (203 Czech Republic)
Edition
Hyderabad, Indie, 3rd International Congress on Bioprocesses in Food Industries (ICBF 2008), p. 47-47, 2008
Publisher
Osmania University, Hyderabad
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
India
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/08:00027098
Organization unit
Faculty of Science
Keywords in English
nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzymes immobilization; molecular diagnostics; PCR
Tags
Tags
International impact
Změněno: 6/1/2009 11:18, doc. Ing. Bohuslav Rittich, CSc.
V originále
Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA with hydrophilic properties were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. For the evaluation of stringent requirements for molecular diagnostics, original PCR application of magnetic microspheres was developed. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNA, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Adsorption of DNA macromolecules on the surface of poly(N-isopropylacrylamide) (PNIPAAm) thermoresponsive particles was also studied.
In Czech
Magnetické mikročástice (P(HEMA-co-EDMA), P(HEMA-co-GMA) a PGMA s hydrofilními vlastnostmi byly připraveny jednostupňovou dispersní polymerizací. Stericky nebo electrostaticky stabilizovaný koloidní magnetit byl připraven srážením Fe2+ a Fe3+ solí a byl enkapsulován dispersní (ko)polymerizací HEMA a GMA. Na mikročástice byly imobilizovány enzymy RNasa A, DNasa I, proteinasa K a protilátky proti buňkám Salmonella. Jejich aktivita byla ověřena pomocí degredace bakteriální RNA, chromosomální a plazmidové DNA, magnetické separace buněk Salmonella nebo degradací jejich intracelulárních inhibitorů PCR. Rovněž byly studována adsorpce makromolekulrní DNA na povrch termoresponzivních poly(N-isopropylakrylamid) (PNIPAAm) částic.
Links
| 1G57037, research and development project |
| ||
| 2B06053, research and development project |
|