Detailed Information on Publication Record
2009
Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis
ZEISBERGEROVÁ, Marta, Andrea ADÁMKOVÁ and Zdeněk GLATZBasic information
Original name
Integration of on-line protein digestion by trypsin in capillary electrophoresis by means of electrophoretically mediated microanalysis
Name in Czech
Integrace tryptického štěpení proteinů do CE pomocí metody EMMA
Authors
ZEISBERGEROVÁ, Marta (203 Czech Republic), Andrea ADÁMKOVÁ (203 Czech Republic) and Zdeněk GLATZ (203 Czech Republic, guarantor)
Edition
Electrophoresis, Weinheim, VHC, 2009, 0173-0835
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.077
RIV identification code
RIV/00216224:14310/09:00029144
Organization unit
Faculty of Science
UT WoS
000268626900017
Keywords in English
Proteomics; Tryptic digestion; Electrophoretically mediated microanalysis; EMMA; Partial filling technique; Sandwich injection
Tags
Tags
International impact, Reviewed
Změněno: 9/4/2010 10:03, Mgr. Marta Pelcová, Ph.D.
V originále
In this work, electrophoretically mediated microanalysis (EMMA) was applied to the in-capillary tryptic digestion of proteins for proteomic purposes. Compared to classical in-solution tryptic digestion or the trypsin reactor commonly used for this purpose, the EMMA based method is rapid, can be automated and requires only small amounts of trypsin and protein preparations. Moreover, the protein digestion and the analysis of the resulting peptides are integrated into one procedure. A combination of the EMMA methodology with a partial filling technique was used in this study, since the pH optimum of the trypsin reaction differs strongly from the best pH for the CE separation of peptides. In this setup, part of the capillary is filled with the best buffer for the tryptic digestion (50 mM Tris-HCl buffer, pH 8.5) whereas the rest of the capillary is filled with the background electrolyte optimal for peptide separation (0.1 M phosphate buffer, pH 2.5).
In Czech
Byla vypracována metoda pro integrace tryptického štěpení proteinů do CE pomocí metody EMMA.
Links
| GA203/06/0047, research and development project |
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| LC06023, research and development project |
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| MSM0021622413, plan (intention) |
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