Functional analysis of the aglycone - binding site of the maize beta-glucosidase Zm-p60.1

DOPITOVÁ, Radka, Pavel MAZURA, Lubomír JANDA, Radka CHALOUPKOVÁ, Petr JEŘÁBEK, Jiří DAMBORSKÝ, Tomáš FILIPI, Nagavalli S. KIRAN and Břetislav BRZOBOHATÝ. Functional analysis of the aglycone - binding site of the maize beta-glucosidase Zm-p60.1. FEBS Journal. 2008, vol. 275, No 24, p. 6123-6135. ISSN 1742-464X.

Basic information

• Original name: Functional analysis of the aglycone - binding site of the maize beta-glucosidase Zm-p60.1
• Name in Czech: Funkční analýza aglykon vazebného místa kukuřičné beta glukosidasy Zm-p60.1
• Authors: DOPITOVÁ, Radka (203 Czech Republic, belonging to the institution), Pavel MAZURA (203 Czech Republic), Lubomír JANDA (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Petr JEŘÁBEK (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic, belonging to the institution), Tomáš FILIPI (203 Czech Republic), Nagavalli S. KIRAN (356 India) and Břetislav BRZOBOHATÝ (203 Czech Republic, guarantor).
• Edition: FEBS Journal, 2008, 1742-464X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.139
• RIV identification code: RIV/00216224:14310/08:00027235
• Organization unit: Faculty of Science
• UT WoS: 000261184700009
• Keywords in English: aglycone-binding site; Brassica napus; substrate specificity; Zea mays; b-glucosidase
• Tags: aglycone-binding site, B-Glucosidase, Brassica napus, substrate specificity, Zea mays

Abstract

The work deals with the key amino acids determining substrate specificity in b-glucosidase Zm-p60.1. New informations about determination of substrate specificity and effect of key amino acid on function and structure have been obtained on the basis of bioinformational, structural and kinetic analysis and molecular docking data.

Abstract (in Czech)

Práce se zabývá klíčovými aminokyselinami determinujícími vstup do aktivního centra kukuřičné b-glukosidasy Zm-p60.1. Na základě bioinformatické analýzy, strukturních a kinetických dat a údajů molekulárního dokingu byly získány nové poznatky o determinaci substrátové specifity a vlivu klíčových aminokyselin na funkci a strukturu enzymu.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations