Chip-based CE for avidin determination in transgenic tobacco
and its comparison with square-wave voltammetry and standard
gel electrophoresis

J 2008

Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis

KŘÍŽKOVÁ, Soňa, Vendula HRDINOVÁ, Vojtěch ADAM, Elisabeth BURGESS, Karl KRAMER et. al.

Basic information

Original name

Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis

Name in Czech

Čipová kapilární elektroforéza jako metodika pro stanovení avidinu v transgenním tabáku a porovnání se square wave voltametrií a standardní gelovou elektroforézou

Authors

KŘÍŽKOVÁ, Soňa, Vendula HRDINOVÁ, Vojtěch ADAM, Elisabeth BURGESS, Karl KRAMER, Michal MASAŘÍK and René KIZEK

Edition

Chromatographia, Friedrich Vieweg a synové, 2008, 0009-5893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.312

Organization unit

Faculty of Science

UT WoS

000256533700011

Keywords in English

chip-based capillary electrophoresis; avidin; transgenic tobacco plants; voltammetry; SDS-PAGE

Abstract

ORIG CZ

V originále

Avidin transgenic plants are a potential tool for providing resistance against various species of insect pests due to the sequestration of vitamin H (biotin) in the plant from the insect pests. In this project we compared three techniques for avidin determination in transgenic tobacco plants, a novel chip-based capillary electrophoretic method (Experion), classical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE) and a square wave voltammetric method using a carbon paste electrode. We determined that the automated chip-based capillary electrophoretic method is rapid, sensitive and the results obtained are well repeatable. The avidin content measured in transgenic tobacco leaves using chip-based capillary electrophoresis varied from 15 to 854 ng per mg of fresh mass depending on the individual plant.

In Czech

Avidin v transgenních rostlinách je potenciálním biopesticidem proti řadě škůdců. V této práci jsme porovnávali tři techniky stanovení avidinu z transgenních rostlin - novou čipovou kapilární elektroforézu, dále klasickou polyakrylamidovou gelovou elektroforézu a elektrochemickou analýzu. Zjistili jsme, že čipová kapilární elektroforéza je rychlá a citlivá metoda a poskytuje reprodukovatelné výsledky.

Citovat

KŘÍŽKOVÁ, Soňa, Vendula HRDINOVÁ, Vojtěch ADAM, Elisabeth BURGESS, Karl KRAMER, Michal MASAŘÍK and René KIZEK. Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis. Chromatographia. Friedrich Vieweg a synové, 2008, vol. 67, Suppl. S, p. S75-S81, 7 pp. ISSN 0009-5893.

@article{807057,
   author = {Křížková, Soňa and Hrdinová, Vendula and Adam, Vojtěch and Burgess, Elisabeth and Kramer, Karl and Masařík, Michal and Kizek, René},
   article_number = {Suppl. S},
   keywords = {chip-based capillary electrophoresis; avidin; transgenic tobacco plants; voltammetry; SDS-PAGE},
   language = {eng},
   issn = {0009-5893},
   journal = {Chromatographia},
   title = {Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis},
   volume = {67},
   year = {2008}
}

TY  - JOUR
ID  - 807057
AU  - Křížková, Soňa - Hrdinová, Vendula - Adam, Vojtěch - Burgess, Elisabeth - Kramer, Karl - Masařík, Michal - Kizek, René
PY  - 2008
TI  - Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis
JF  - Chromatographia
VL  - 67
IS  - Suppl. S
SP  - S75-S81
EP  - S75-S81
PB  - Friedrich Vieweg a synové
SN  - 00095893
KW  - chip-based capillary electrophoresis
KW  - avidin
KW  - transgenic tobacco plants
KW  - voltammetry
KW  - SDS-PAGE
N2  - Avidin transgenic plants are a potential tool for providing resistance against various species of insect pests due to the sequestration of vitamin H (biotin) in the plant from the insect pests. In this project we compared three techniques for avidin determination in transgenic tobacco plants, a novel chip-based capillary electrophoretic method (Experion), classical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE) and a square wave voltammetric method using a carbon paste electrode. We determined that the automated chip-based capillary electrophoretic method is rapid, sensitive and the results obtained are well repeatable. The avidin content measured in transgenic tobacco leaves using chip-based capillary electrophoresis varied from 15 to 854 ng per mg of fresh mass depending on the individual plant.
ER  -

KŘÍŽKOVÁ, Soňa, Vendula HRDINOVÁ, Vojtěch ADAM, Elisabeth BURGESS, Karl KRAMER, Michal MASAŘÍK and René KIZEK. Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis. \textit{Chromatographia}. Friedrich Vieweg a synové, 2008, vol.~67, Suppl. S, p.~S75-S81, 7 pp. ISSN~0009-5893.

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