| LOCHMANOVÁ, Gabriela, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Šárka KOUKALOVÁ, Jiří MALBECK, Přemysl SOUČEK, Martina VÁLKOVÁ, Nagavalli KIRAN and Břetislav BRZOBOHATÝ. Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis. Journal of Experimental Botany. 2008, vol. 59, No 13, p. 3705-3719. ISSN 0022-0957. |
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@article{807149,
author = {Lochmanová, Gabriela and Zdráhal, Zbyněk and Konečná, Hana and Koukalová, Šárka and Malbeck, Jiří and Souček, Přemysl and Válková, Martina and Kiran, Nagavalli and Brzobohatý, Břetislav},
article_number = {13},
keywords = {Arabidopsis thaliana; cytokinin; 2D electrophoresis; photomorphogenesis; proteome},
language = {eng},
issn = {0022-0957},
journal = {Journal of Experimental Botany},
title = {Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis},
volume = {59},
year = {2008}
}
TY - JOUR
ID - 807149
AU - Lochmanová, Gabriela - Zdráhal, Zbyněk - Konečná, Hana - Koukalová, Šárka - Malbeck, Jiří - Souček, Přemysl - Válková, Martina - Kiran, Nagavalli - Brzobohatý, Břetislav
PY - 2008
TI - Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis
JF - Journal of Experimental Botany
VL - 59
IS - 13
SP - 3705-3719
EP - 3705-3719
SN - 00220957
KW - Arabidopsis thaliana
KW - cytokinin
KW - 2D electrophoresis
KW - photomorphogenesis
KW - proteome
N2 - The effects on the proteome of increased levels of endogenous CKs in dark grown Arabidopsis seedlings were studied. Increase in endogenous CK levels was achieved by inducing the CK biosynthetic gene, ipt. Image and mass spectrometric analysis of proteins separated in two dimensional gels identified a number of differentially expressed proteins, most of which are related to chloroplast biogenesis and function. All the identified chloroplast proteins were upregulated in response to increased CK levels. In contrast, all differentially regulated mitochondrial proteins, representing the second largest protein group, were downregulated. Comparison of our proteomic data with previously published transcriptome profiling of light induced photomorphogenesis and effects of increased CK levels in light grown Arabidopsis seedlings revealed the same general trends for most of the 23 transcripts corresponding to the proteins in our data set.
ER -
LOCHMANOVÁ, Gabriela, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Šárka KOUKALOVÁ, Jiří MALBECK, Přemysl SOUČEK, Martina VÁLKOVÁ, Nagavalli KIRAN and Břetislav BRZOBOHATÝ. Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis. \textit{Journal of Experimental Botany}. 2008, vol.~59, No~13, p.~3705-3719. ISSN~0022-0957.
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