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@article{808467,
author = {Řepková, Jana and Dreiseitl, Antonín and Lízal, Pavel},
article_location = {Maďarsko},
article_number = {1},
keywords = {Blumeria graminis f. sp. hordei; DNA markers; Hordeum vulgare ssp. spontaneum; resistance gene homolog},
language = {eng},
issn = {0133-3720},
journal = {Cereal Research Communications},
title = {New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus},
volume = {37},
year = {2009}
}
TY - JOUR
ID - 808467
AU - Řepková, Jana - Dreiseitl, Antonín - Lízal, Pavel
PY - 2009
TI - New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus
JF - Cereal Research Communications
VL - 37
IS - 1
SP - 93-99
EP - 93-99
PB - Akademiai Kiado
SN - 01333720
KW - Blumeria graminis f. sp. hordei
KW - DNA markers
KW - Hordeum vulgare ssp. spontaneum
KW - resistance gene homolog
N2 - In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the development of a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria graminis f. sp. hordei) and plants homozygous or heterozygous for the resistance allele. The RGH1aI1a marker was positioned 0.85 cM to the resistance gene and the efficiency of marker-assisted selection (MAS), evaluated as the probability of crossing-over between the marker and the targeted gene, was 99%. The CAPS marker RGH1aI1a is a valuable candidate for MAS and gene transfer into barley varieties susceptible to powdery mildew.
ER -
ŘEPKOVÁ, Jana, Antonín DREISEITL and Pavel LÍZAL. New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus. \textit{Cereal Research Communications}. Maďarsko: Akademiai Kiado, 2009, vol.~37, No~1, p.~93-99. ISSN~0133-3720.
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