Determination of capsidiol in tobacco cells culture by HPLC

LITERÁKOVÁ, Petra, Jan LOCHMAN, Zbyněk ZDRÁHAL, Zbyněk PROKOP, Vladimír MIKEŠ and Tomáš KAŠPAROVSKÝ. Determination of capsidiol in tobacco cells culture by HPLC. Journal of Chromatographic Science. 2010, vol. 48, No 6, p. 436-440. ISSN 0021-9665.

Basic information

• Original name: Determination of capsidiol in tobacco cells culture by HPLC
• Name in Czech: Stanovení capsidiolu v kalusových kulturách tabáku pomocí HPLC
• Authors: LITERÁKOVÁ, Petra (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Vladimír MIKEŠ (203 Czech Republic, belonging to the institution) and Tomáš KAŠPAROVSKÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Journal of Chromatographic Science, 2010, 0021-9665.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.078
• RIV identification code: RIV/00216224:14310/10:00042276
• Organization unit: Faculty of Science
• UT WoS: 000280253700003
• Keywords in English: Capsidiol Tobacco HPLC
• Tags: Capsidiol Tobacco HPLC
• Tags: International impact, Reviewed

Abstract

Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. The relative standard deviation across five extractions of known amounts of capsidiol from plant sample was less than 5.1%. The relative standard deviation across five elicitations of cell cultures was less than 5.9%. GC-MS and LC-MS analysis of capsidiol was performed and corresponding MS spectra are presented.

Abstract (in Czech)

Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. The relative standard deviation across five extractions of known amounts of capsidiol from plant sample was less than 5.1%. The relative standard deviation across five elicitations of cell cultures was less than 5.9%. GC-MS and LC-MS analysis of capsidiol was performed and corresponding MS spectra are presented.

Links

• GA522/06/0156, research and development project: Name: Vztah mezi strukturou a funkcí elicitoru Phytophthora spp.

Investor: Czech Science Foundation, Structure-function relationship of elicitors from Phytophthora spp.

• ME 942, research and development project: Name: Nové metody biologické kontroly infekce rostlin

Investor: Ministry of Education, Youth and Sports of the CR, New methods of biological control of plant disease

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations