| PRAKASH, Rohit, D. SATORY, E. DRAY, A. PAPUSHA, J. SCHELLER, W. KRAMER, Lumír KREJČÍ, Hannah KLEIN, J.E. HABER, Patrick SUNG and Grzegorz IRA. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev. 2009, vol. 23, No 1, p. 67-79, 12 pp. ISSN 1549-5477. |
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@article{815037,
author = {Prakash, Rohit and Satory, D. and Dray, E. and Papusha, A. and Scheller, J. and Kramer, W. and Krejčí, Lumír and Klein, Hannah and Haber, J.E. and Sung, Patrick and Ira, Grzegorz},
article_number = {1},
keywords = {Genome instability; recombination; DNA helicase; crossing over; Fanconi anemia},
language = {eng},
issn = {1549-5477},
journal = {Genes Dev.},
title = {Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.},
volume = {23},
year = {2009}
}
TY - JOUR
ID - 815037
AU - Prakash, Rohit - Satory, D. - Dray, E. - Papusha, A. - Scheller, J. - Kramer, W. - Krejčí, Lumír - Klein, Hannah - Haber, J.E. - Sung, Patrick - Ira, Grzegorz
PY - 2009
TI - Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
JF - Genes Dev.
VL - 23
IS - 1
SP - 67-79
EP - 67-79
SN - 15495477
KW - Genome instability
KW - recombination
KW - DNA helicase
KW - crossing over
KW - Fanconi anemia
N2 - Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks.
ER -
PRAKASH, Rohit, D. SATORY, E. DRAY, A. PAPUSHA, J. SCHELLER, W. KRAMER, Lumír KREJČÍ, Hannah KLEIN, J.E. HABER, Patrick SUNG and Grzegorz IRA. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. \textit{Genes Dev.}. 2009, vol.~23, No~1, p.~67-79, 12 pp. ISSN~1549-5477.
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