Interaction of cryptogein with its binding sites in tobacco plasma

SVOZILOVÁ, Zuzana, Tomáš KAŠPAROVSKÝ, Petr SKLÁDAL and Jan LOCHMAN. Interaction of cryptogein with its binding sites in tobacco plasma. Analytical biochemistry. 2009, vol. 390, No 2, p. 115-120. ISSN 0003-2697.

Basic information

• Original name: Interaction of cryptogein with its binding sites in tobacco plasma
• Name in Czech: Interakce crytpogeinu s jeho vazabným místem na plazmatické membráně tabáku
• Authors: SVOZILOVÁ, Zuzana (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Analytical biochemistry, 2009, 0003-2697.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.287
• RIV identification code: RIV/00216224:14310/09:00029172
• Organization unit: Faculty of Science
• UT WoS: 000266946000003
• Keywords in English: Membrane receptor Membrane vesicles Real time bioanalysis Elicitins Kinetic
• Tags: International impact, Reviewed

Abstract

Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.

Abstract (in Czech)

Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.

Links

• GA522/06/0156, research and development project: Name: Vztah mezi strukturou a funkcí elicitoru Phytophthora spp.

Investor: Czech Science Foundation, Structure-function relationship of elicitors from Phytophthora spp.

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment