| ŠPANOVÁ, Alena, Jitka HERZOGOVÁ and Bohuslav RITTICH. Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR. In 4th Meeting on Chemistry and Life, Chemické listy,102 (2008) No15. Brno: VUT Brno, Chemická fakulta, 2008, p. 791-792. ISSN 0009-2770. |
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@inproceedings{815664,
author = {Španová, Alena and Herzogová, Jitka and Rittich, Bohuslav},
address = {Brno},
booktitle = {4th Meeting on Chemistry and Life, Chemické listy,102 (2008) No15},
keywords = {cheese; Lactococcus lactis; P(HEMA-co-EDMA); PCR;},
language = {eng},
location = {Brno},
pages = {791-792},
publisher = {VUT Brno, Chemická fakulta},
title = {Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR},
year = {2008}
}
TY - JOUR
ID - 815664
AU - Španová, Alena - Herzogová, Jitka - Rittich, Bohuslav
PY - 2008
TI - Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR
PB - VUT Brno, Chemická fakulta
CY - Brno
KW - cheese
KW - Lactococcus lactis
KW - P(HEMA-co-EDMA)
KW - PCR;
N2 - PCR-ready DNA was isolated from different type of cheese using magnetic microspheres P(HEMA-co-EDMA) containing carboxyl groups in the presence of high concentrations of PEG 6000 and NaCl. The quality of extracted DNA was checked by PCR amplification. Species-specific PCR primers PALA4 and PALA14 (targeted on acm A gene encoding N-acetylmuramidase specific to Lactococcus lactis, 1131 bp long PCR products ) were used for the identification of Lactococcus lactis species with success.
ER -
ŠPANOVÁ, Alena, Jitka HERZOGOVÁ and Bohuslav RITTICH. Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR. In \textit{4th Meeting on Chemistry and Life, Chemické listy,102 (2008) No15}. Brno: VUT Brno, Chemická fakulta, 2008, p.~791-792. ISSN~0009-2770.
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