PALEČEK, Emil, Marie BRÁZDOVÁ, Hana ČERNOCKÁ, Daniel VLK, Václav BRÁZDA and Bořivoj VOJTĚŠEK. Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments. Oncogene. Basingstoke: Macmillan Press, vol. 24, No 18, p. 3617-3625. ISSN 0950-9232. 1999.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Effect of transition metals on binding of p53 protein to supercoiled DNA and to consensus sequence in DNA fragments
Authors PALEČEK, Emil, Marie BRÁZDOVÁ, Hana ČERNOCKÁ, Daniel VLK, Václav BRÁZDA and Bořivoj VOJTĚŠEK.
Edition Oncogene, Basingstoke, Macmillan Press, 1999, 0950-9232.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 6.517
Organization unit Faculty of Science
Keywords in English p53; DNA-p53 interaction; scDNA; divalent cation; gel electrophoresis
Tags divalent cation, DNA-p53 interaction, gel electrophoresis, p53, scDNA
Tags International impact, Reviewed
Changed by Changed by: Mgr. Daniel Vlk, CSc., učo 98282. Changed: 22/1/2009 13:07.
Abstract
Recently we have shown that wild-type human p53 protein binds preferentially to supercoiled (sc) DNA in vitro in both the presence and absence of the p53 consensus sequence (p53CON). This binding produces a ladder of retarded bands on an agarose gel. The intensity and the number of these hands are decreased by physiological concentrations of zinc ions. At higher zinc concentrations, binding of p53 to scDNA is completely inhibited.
PrintDisplayed: 16/4/2024 18:44