Detailed Information on Publication Record
2009
The effect of bortezomib on myeloma cell line
POTÁČOVÁ, Anna, Jana ČUMOVÁ, Irena KASALOVÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL et. al.Basic information
Original name
The effect of bortezomib on myeloma cell line
Name in Czech
Vliv bortezomibu na myelomové buňky
Authors
POTÁČOVÁ, Anna, Jana ČUMOVÁ, Irena KASALOVÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL and Roman HÁJEK
Edition
XII. International Myeloma Workshop, 2009
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
ISBN
1557-9190
UT WoS
000268206500217
Keywords (in Czech)
mnohočetný myelom;bortezomib
Keywords in English
multiple myeloma; proteomics;bortezomib
Tags
Změněno: 13/3/2009 14:51, Mgr. Anna Potáčová, Ph.D.
Abstract
V originále
Introduction: Multiple myeloma (MM) is still an incurable disease characterized by the clonal expansion of malignant plasma cells. New anticancer drugs further improve prognosis of myeloma patients. The aim of this study was to evaluate changes in protein expression of myeloma cell line ARH 77 after bortezomib treatment. Materials and methods: Myeloma cell line ARH 77 was treated with bortezomib (10 – 40nM) for various periods of time (24 and 48 hours). The proteins contained in total myeloma cell lysate were separated by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and the differentially expressed proteins between the untreated and treated cell lines were excised and identified by mass spectrometry. Results: There were analyzed 94 proteins differentially expressed between treated and control cells; total of 34 protein spots were upregulated: proteins involved in regulation of apoptosis, chaperons/stress related proteins, proteolysis of ubiquitin/protein degradation and cytoskeleton proteins. Sixty protein spots were downregulated: proteins involved in synthesis, regulation of apoptosis, chaperons/stress related proteins, regulation of cell cycle proteins, proteins connected to glycolysis and proteolysis of ubiquitin/protein degradation and antioxidant/redox proteins. Conclusion: We identified 94 proteins altered in myeloma cells after various exposure of to bortezomib. This proteomic approach can contribute to elucidation of mechanisms of new anticancer drugs action.
Links
| LC06027, research and development project |
| ||
| MSM0021622434, plan (intention) |
|