ŠMAJS, David, Magda DOLEŽALOVÁ, Pavel MACEK and Lukáš ŽÍDEK. Inactivation of colicin Y by intramembrane helix helix interaction with its immunity protein (Inactivation of colicin Y by intramembrane helixhelix interaction with its immunity protein). FEBS Journal. 2008, vol. 275, No 21, p. 5325 - 5331. ISSN 1742-464X.
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Basic information
Original name Inactivation of colicin Y by intramembrane helix helix interaction with its immunity protein
Name in Czech Inaktivace kolicinu Y jeho imunitním proteinem
Authors ŠMAJS, David (203 Czech Republic, guarantor), Magda DOLEŽALOVÁ (203 Czech Republic), Pavel MACEK (203 Czech Republic) and Lukáš ŽÍDEK (203 Czech Republic).
Edition FEBS Journal, 2008, 1742-464X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.139
RIV identification code RIV/00216224:14310/08:00027657
Organization unit Faculty of Science
UT WoS 000260010600008
Keywords in English colicin immunity; colicin Y; helix helix interaction; pore orming colicin; site drected mutagenesis
Tags colicin immunity, colicin Y, helix helix interaction, pore orming colicin, site drected mutagenesis
Tags Reviewed
Changed by Changed by: prof. Mgr. Lukáš Žídek, Ph.D., učo 38990. Changed: 8/6/2010 10:40.
Abstract
Construction of hybrids between colicins U and Y and mutagenesis of colicin Y gene (cya) revealed amino acid residues important for interactions between colicin Y and its cognate immunity protein (Cyi). Four such residues (I578, T582, Y586, and V590) were found in helices 8 and 9 of the colicin Y pore forming domain. To verify the importance of these residues, the corresponding amino acids in the colicin B protein were mutated to the residues present in colicin Y. An E. coli strain with cloned colicin Y immunity gene (cyi) inactivated this mutant but not the wildtype colicin B. In addition, interacting amino acid pairs in Cya and Cyi were identified using a set of Cyi point mutant strains. These data are consistent with antiparallel helix-helix interactions between Cyi helix T3 and Cya helix 8 of the pore-forming domain as a molecular mechanism of colicin Y inactivation by its immunity protein.
Abstract (in Czech)
Je popsána inaktivace kolicinu Y jeho imunitním proteinem, predevším pak jde o identifikaci aminokyselinových zbyků, které se podílí na interakci mezi kolicinem a jeho imunitním proteinem.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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