Capillary immunotyping electrophoresis and high resolution
two-dimensional electrophoresis for the detection of mu-heavy
chain disease.

J 2008

Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease.

MAISNAR, Vladimír, Miloš TICHÝ, J. STULÍK, P. URBAN, Zdeněk ADAM et. al.

Basic information

Original name

Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease.

Name in Czech

Užití kapilární a dvourozměrné elektroforézy pro stanovení mu těžkých řetězců

Authors

MAISNAR, Vladimír (203 Czech Republic), Miloš TICHÝ (203 Czech Republic), J. STULÍK (203 Czech Republic), P. URBAN (203 Czech Republic), Zdeněk ADAM (203 Czech Republic, guarantor), Eva KDLČKOVÁ (203 Czech Republic), J. VÁVROVÁ (203 Czech Republic), Vladimír PALIČKA (203 Czech Republic), L. JEBAVÝ (203 Czech Republic), R. KODET (203 Czech Republic), Tomáš BÜCHLER (203 Czech Republic) and Roman HÁJEK (203 Czech Republic)

Edition

Clinica Chimica Acta, Amsterdam, Elsevier Science BV, 2008, 0009-8981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.960

RIV identification code

RIV/00216224:14110/08:00034898

Organization unit

Faculty of Medicine

UT WoS

000253450300030

Keywords (in Czech)

mnohočetný myelom; monoklonální imunoglobulin;imunofixace

Keywords in English

multiple myeloma;monoclonal immunoglobulin;immunofixation

Abstract

ORIG CZ

V originále

Heavy chain disease with monoclonal incomplete mu-heavy chains (mu-HCD) is a rare disorder usually associated with an underlying lymphoproliferative malignancy. Laboratory diagnosis of patients with mu-HCD is usually challenging and the monoclonal protein is not detected by electrophoresis in up to 75% of mu-HCD cases. We describe a patient with multiple malignancies in whom we detected and characterized monoclonal mu-heavy chains using immunofixation electrophoresis, capillary zone electrophoresis with immunotyping, and high resolution two-dimensional electrophoresis. The high resolution 2D electrophoresis enabled us to determine the molecular weight of the mu-heavy chains. The abnormal protein concentration in the serum was unusually high, 38 g/l measured in our patient is the highest reported value in the literature so far.

In Czech

V této práce je popsáno užití kapilární a dvourozměrné elektroforézy pro stanovení mu těžkých řetězců.

Citovat

MAISNAR, Vladimír, Miloš TICHÝ, J. STULÍK, P. URBAN, Zdeněk ADAM, Eva KDLČKOVÁ, J. VÁVROVÁ, Vladimír PALIČKA, L. JEBAVÝ, R. KODET, Tomáš BÜCHLER and Roman HÁJEK. Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease. Clinica Chimica Acta. Amsterdam: Elsevier Science BV, 2008, vol. 389, 1-2, p. 171-173. ISSN 0009-8981.

@article{817663,
   author = {Maisnar, Vladimír and Tichý, Miloš and Stulík, J. and Urban, P. and Adam, Zdeněk and Kdlčková, Eva and Vávrová, J. and Palička, Vladimír and Jebavý, L. and Kodet, R. and Büchler, Tomáš and Hájek, Roman},
   article_location = {Amsterdam},
   article_number = {1-2},
   keywords = {multiple myeloma;monoclonal immunoglobulin;immunofixation},
   language = {eng},
   issn = {0009-8981},
   journal = {Clinica Chimica Acta},
   title = {Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease.},
   volume = {389},
   year = {2008}
}

TY  - JOUR
ID  - 817663
AU  - Maisnar, Vladimír - Tichý, Miloš - Stulík, J. - Urban, P. - Adam, Zdeněk - Kdlčková, Eva - Vávrová, J. - Palička, Vladimír - Jebavý, L. - Kodet, R. - Büchler, Tomáš - Hájek, Roman
PY  - 2008
TI  - Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease.
JF  - Clinica Chimica Acta
VL  - 389
IS  - 1-2
SP  - 171-173
EP  - 171-173
PB  - Elsevier Science BV
SN  - 00098981
KW  - multiple myeloma;monoclonal immunoglobulin;immunofixation
N2  - Heavy chain disease with monoclonal incomplete mu-heavy chains (mu-HCD) is a rare disorder usually associated with an underlying lymphoproliferative malignancy. Laboratory diagnosis of patients with mu-HCD is usually challenging and the monoclonal protein is not detected by electrophoresis in up to 75% of mu-HCD cases. We describe a patient with multiple malignancies in whom we detected and characterized monoclonal mu-heavy chains using immunofixation electrophoresis, capillary zone electrophoresis with immunotyping, and high resolution two-dimensional electrophoresis. The high resolution 2D electrophoresis enabled us to determine the molecular weight of the mu-heavy chains. The abnormal protein concentration in the serum was unusually high, 38 g/l measured in our patient is the highest reported value in the literature so far.
ER  -

MAISNAR, Vladimír, Miloš TICHÝ, J. STULÍK, P. URBAN, Zdeněk ADAM, Eva KDLČKOVÁ, J. VÁVROVÁ, Vladimír PALIČKA, L. JEBAVÝ, R. KODET, Tomáš BÜCHLER and Roman HÁJEK. Capillary immunotyping electrophoresis and high resolution two-dimensional electrophoresis for the detection of mu-heavy chain disease. \textit{Clinica Chimica Acta}. Amsterdam: Elsevier Science BV, 2008, vol.~389, 1-2, p.~171-173. ISSN~0009-8981.

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