Antioxidant lignans from petroleum ether extract of Schisandra
chinensis

J 2008

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ and Otakar HUMPA

Basic information

Original name

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

Name in Czech

Antioxidační lignany z petroletherového extraktu Schisandra chinensis

Authors

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ and Otakar HUMPA

Edition

Planta Medica, Stuttgart - New York, Thieme Medical Publishers, 2008, 0032-0943

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.960

Organization unit

Faculty of Medicine

UT WoS

000258261800209

Keywords in English

Schisandra chinensis; lignans; DPPH; HPLC

Abstract

ORIG CZ

V originále

The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of viral and chemically induced hepatitis in China. Extracts of Schisandra chinensis have demonstrated hepatoprotective and cardioprotective activities, which can be potentially connected with ability to reduce oxidative stress. The aim of our study was to characterise compounds, which correspond for antioxidant activity of Schisandra chinensis. The petroleum ether extract of seeds of Schisandra chinensis showed scavenging activity against the free stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The petroleum ether extract was extracted with methanol and the methanolic extract was further chromatographed on silica gel column with petroleum ether, benzene, diethyl ether and ethanol as a mobile phase. The most active fractions eluted with benzene were further fractioned on silica gel column and Sephadex LH-20 column and by semi-preparative RP-HPLC. Bioassay guided fractionation led to the isolation of dibenzylbutane lignan meso-dihydroguaiaretic acid and dibenzo[a,c]cyclooctadiene lignans schisanhenol and gomisin J. All active lignans containing one or two phenolic hydroxyls belong to minor lignan constituents of Schisandra. Together with the active lignans, nine dibenzocyclooctadiene lignans were isolated, namely, schizandrin, deoxyschizandrin, gamma-schizandrin, gomisin A, gomisin N, tigloylgomisin H, angeloylgomisin H, tigloylgomisin P and wuweizisu C. The lignans were identified by spectroscopic methods (1H and 13C NMR, MS).

In Czech

Antioxidační lignany, meso-dihydrogvajaretová kyselina,schisanhenol a gomisin J z petroletherového extraktu Schisandra chinensis byly vyizolovány.

Links

GA522/07/0995, research and development project

Name: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis

Investor: Czech Science Foundation

Citovat

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ and Otakar HUMPA. Antioxidant lignans from petroleum ether extract of Schisandra chinensis. Planta Medica. Stuttgart - New York: Thieme Medical Publishers, 2008, vol. 74, No 9, p. 952-952. ISSN 0032-0943.

@article{818309,
   author = {Slanina, Jiří and Březinová, Lenka and Paulová, Hana and Humpa, Otakar},
   article_location = {Stuttgart - New York},
   article_number = {9},
   keywords = {Schisandra chinensis; lignans; DPPH; HPLC},
   language = {eng},
   issn = {0032-0943},
   journal = {Planta Medica},
   title = {Antioxidant lignans from petroleum ether extract of Schisandra chinensis},
   volume = {74},
   year = {2008}
}

TY  - JOUR
ID  - 818309
AU  - Slanina, Jiří - Březinová, Lenka - Paulová, Hana - Humpa, Otakar
PY  - 2008
TI  - Antioxidant lignans from petroleum ether extract of Schisandra chinensis
JF  - Planta Medica
VL  - 74
IS  - 9
SP  - 952-952
EP  - 952-952
PB  - Thieme Medical Publishers
SN  - 00320943
KW  - Schisandra chinensis
KW  - lignans
KW  - DPPH
KW  - HPLC
N2  - The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of viral and chemically induced hepatitis in China. Extracts of Schisandra chinensis have demonstrated hepatoprotective and cardioprotective activities, which can be potentially connected with ability to reduce oxidative stress. The aim of our study was to characterise compounds, which correspond for antioxidant activity of Schisandra chinensis. The petroleum ether extract of seeds of Schisandra chinensis showed scavenging activity against the free stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The petroleum ether extract was extracted with methanol and the methanolic extract was further chromatographed on silica gel column with petroleum ether, benzene, diethyl ether and ethanol as a mobile phase. The most active fractions eluted with benzene were further fractioned on silica gel column and Sephadex LH-20 column and by semi-preparative RP-HPLC. Bioassay guided fractionation led to the isolation of dibenzylbutane lignan meso-dihydroguaiaretic acid and dibenzo[a,c]cyclooctadiene lignans schisanhenol and gomisin J. All active lignans containing one or two phenolic hydroxyls belong to minor lignan constituents of Schisandra. Together with the active lignans, nine dibenzocyclooctadiene lignans were isolated, namely, schizandrin, deoxyschizandrin, gamma-schizandrin, gomisin A, gomisin N, tigloylgomisin H, angeloylgomisin H, tigloylgomisin P and wuweizisu C. The lignans were identified by spectroscopic methods (1H and 13C NMR, MS).
ER  -

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ and Otakar HUMPA. Antioxidant lignans from petroleum ether extract of Schisandra chinensis. \textit{Planta Medica}. Stuttgart - New York: Thieme Medical Publishers, 2008, vol.~74, No~9, p.~952-952. ISSN~0032-0943.

Detailed Information on Publication Record