Detailed Information on Publication Record
2009
Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry
SVOBODA, David, Michal KOZUBEK and Stanislav STEJSKALBasic information
Original name
Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry
Name in Czech
Generování digitálních fantomů buněčných jader a simulace tvorby obrazu v 3D obrazové cytometrii
Authors
SVOBODA, David (203 Czech Republic, guarantor, belonging to the institution), Michal KOZUBEK (203 Czech Republic, belonging to the institution) and Stanislav STEJSKAL (203 Czech Republic, belonging to the institution)
Edition
Cytometry Part A, John Wiley & Sons, Inc. 2009, 1552-4922
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.032
RIV identification code
RIV/00216224:14330/09:00034932
Organization unit
Faculty of Informatics
UT WoS
000266470100003
Keywords in English
digital phantom; synthetic image; procedural texture; point spread function; fluorescence optical microscope; 3D image cytometry
Tags
Tags
International impact, Reviewed
Změněno: 19/12/2016 17:46, prof. RNDr. Michal Kozubek, Ph.D.
V originále
Image cytometry still faces the problem of the quality of cell image analysis results. Degradations caused by cell preparation, optics and electronics considerably affect most 2D and 3D cell image data acquired using optical microscopy. That is why image processing algorithms applied to these data typically offer imprecise and unreliable results. We have created a toolbox that can generate 3D digital phantoms of specific cellular components along with their corresponding images degraded by specific optics and electronics. The user can then apply image analysis methods to such simulated image data. The analysis results can be compared with ground truth derived from input object digital phantoms. In this way, image analysis methods can be compared to each other and their quality can be computed. We have also evaluated the plausibility of the synthetic images, measured by their similarity to real image data.
In Czech
V obrazové cytometrii se denně setkáváme s problémem kvality snímků buněk a zejména s problémem kvality analyzačních metod. Jedním ze způsobů měření kvality těchto metod je využití tzv. fantomů. V tomto článku je popsán postup, jakým způsobem vygenerovat digiální fantomy pro specifické druhy biomedicínských dat. Dále se zde také popsána simulace průchodu obrazu takového fantomu optickou soustavou a simulace snímání. Celkově je zde definován popis, jakým způsobem je možné generovat umělá obrazová data. Součástí textu je také měření kvality takto vygenerovaných dat a jejich srovnání s původními realnými obrazovými daty.
Links
| LC535, research and development project |
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| 2B06052, research and development project |
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