| SVOBODA, David, Michal KOZUBEK and Stanislav STEJSKAL. Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry. Cytometry Part A. John Wiley & Sons, Inc., 2009, 75A, No 6, p. 494-509. ISSN 1552-4922. Available from: https://dx.doi.org/10.1002/cyto.a.20714. |
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@article{818483,
author = {Svoboda, David and Kozubek, Michal and Stejskal, Stanislav},
article_number = {6},
doi = {http://dx.doi.org/10.1002/cyto.a.20714},
keywords = {digital phantom; synthetic image; procedural texture; point spread function; fluorescence optical microscope; 3D image cytometry},
language = {eng},
issn = {1552-4922},
journal = {Cytometry Part A},
title = {Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry},
volume = {75A},
year = {2009}
}
TY - JOUR
ID - 818483
AU - Svoboda, David - Kozubek, Michal - Stejskal, Stanislav
PY - 2009
TI - Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry
JF - Cytometry Part A
VL - 75A
IS - 6
SP - 494-509
EP - 494-509
PB - John Wiley & Sons, Inc.
SN - 15524922
KW - digital phantom
KW - synthetic image
KW - procedural texture
KW - point spread function
KW - fluorescence optical microscope
KW - 3D image cytometry
N2 - Image cytometry still faces the problem of the quality of cell image analysis results. Degradations caused by cell preparation, optics and electronics considerably affect most 2D and 3D cell image data acquired using optical microscopy. That is why image processing algorithms applied to these data typically offer imprecise and unreliable results. We have created a toolbox that can generate 3D digital phantoms of specific cellular components along with their corresponding images degraded by specific optics and electronics. The user can then apply image analysis methods to such simulated image data. The analysis results can be compared with ground truth derived from input object digital phantoms. In this way, image analysis methods can be compared to each other and their quality can be computed. We have also evaluated the plausibility of the synthetic images, measured by their similarity to real image data.
ER -
SVOBODA, David, Michal KOZUBEK and Stanislav STEJSKAL. Generation of Digital Phantoms of Cell Nuclei and Simulation of Image Formation in 3D Image Cytometry. \textit{Cytometry Part A}. John Wiley \&{} Sons, Inc., 2009, 75A, No~6, p.~494-509. ISSN~1552-4922. Available from: https://dx.doi.org/10.1002/cyto.a.20714.
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