Detailed Information on Publication Record
2008
Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond.
SLANINOVÁ, Iva, Jiří SLANINA and Eva TÁBORSKÁBasic information
Original name
Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond.
Name in Czech
Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond.
Name (in English)
Fluorescence properties of quaternary benzo[c]fenanthridine alkaloids and their use as a supravital DNA probes.Chemické
Authors
SLANINOVÁ, Iva (203 Czech Republic, guarantor), Jiří SLANINA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic)
Edition
Chemické listy, Praha, Česká společnost chemická, 2008, 0009-2770
Other information
Language
Czech
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.593
RIV identification code
RIV/00216224:14110/08:00025258
Organization unit
Faculty of Medicine
UT WoS
000257129500004
Keywords in English
benzo[c]phenanthridine alkaloids; macarpin; cell cycle; DNA probe
Tags
International impact, Reviewed
Změněno: 22/6/2009 11:51, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Práce popisuje možnost uplatnění qvartérních benzo(c)fenantridinových alkaloidů, především makarpinu, jako supravitálních fluorescenčních DNA sond vhodných pro fluorwscenční mikroskopii a průtokovou cytometrii.
In English
Quaternary benzo[c]phenanthridine alkaloids (QBAs) are natural products isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. They are intensively studied for their biological activities. Nevertheless they have also attractive fluorescence properties. Chromophores responsible for fluorescence are fused aromatic rings with electron-donor groups containing oxygen (OH; OCH3; OCH2O). Recently we have described fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. All these alkaloids immediately enter living cells and MA, CHR and SA bound DNA and showed nuclear architecture similar as common DNA dyes. Moreover, MA binds DNA stoichiometrically and can rapidly report the cellular DNA content in living cells at a resolution level adequate for cell cycle analysis. QBAs were excitable by common argon lasers (488 nm) emitting at the range 575 - 755 nm (i.e. fluorescence detectors FL2-5). Spectral characteristics of MA allow simultaneous surface immunophenotyping. These characteristics allow multiple applications of the above-mentioned QBAs with significant diagnostic utility. They can be used as supravital fluorescent DNA probes both in fluorescence microscopy and flow cytometry including multiparameter analysis.
Links
| GA525/08/0819, research and development project |
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| LC06077, research and development project |
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| MSM0021622415, plan (intention) |
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