Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako ...
SLANINOVÁ, Iva, Jiří SLANINA and Eva TÁBORSKÁ. Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond. (Fluorescence properties of quaternary benzo[c]fenanthridine alkaloids and their use as a supravital DNA probes.Chemické). Chemické listy. Praha: Česká společnost chemická, 2008, vol. 102, No 102, p. 427-433. ISSN 0009-2770. |
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Basic information | |
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Original name | Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond. |
Name in Czech | Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond. |
Name (in English) | Fluorescence properties of quaternary benzo[c]fenanthridine alkaloids and their use as a supravital DNA probes.Chemické |
Authors | SLANINOVÁ, Iva (203 Czech Republic, guarantor), Jiří SLANINA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic). |
Edition | Chemické listy, Praha, Česká společnost chemická, 2008, 0009-2770. |
Other information | |
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Original language | Czech |
Type of outcome | Article in a journal |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Czech Republic |
Confidentiality degree | is not subject to a state or trade secret |
Impact factor | Impact factor: 0.593 |
RIV identification code | RIV/00216224:14110/08:00025258 |
Organization unit | Faculty of Medicine |
UT WoS | 000257129500004 |
Keywords in English | benzo[c]phenanthridine alkaloids; macarpin; cell cycle; DNA probe |
Tags | Benzo[c]phenanthridine Alkaloids, cell cycle, DNA probe, macarpin |
Tags | International impact, Reviewed |
Changed by | Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 22/6/2009 11:51. |
Abstract |
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Práce popisuje možnost uplatnění qvartérních benzo(c)fenantridinových alkaloidů, především makarpinu, jako supravitálních fluorescenčních DNA sond vhodných pro fluorwscenční mikroskopii a průtokovou cytometrii. |
Abstract (in English) |
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Quaternary benzo[c]phenanthridine alkaloids (QBAs) are natural products isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. They are intensively studied for their biological activities. Nevertheless they have also attractive fluorescence properties. Chromophores responsible for fluorescence are fused aromatic rings with electron-donor groups containing oxygen (OH; OCH3; OCH2O). Recently we have described fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. All these alkaloids immediately enter living cells and MA, CHR and SA bound DNA and showed nuclear architecture similar as common DNA dyes. Moreover, MA binds DNA stoichiometrically and can rapidly report the cellular DNA content in living cells at a resolution level adequate for cell cycle analysis. QBAs were excitable by common argon lasers (488 nm) emitting at the range 575 - 755 nm (i.e. fluorescence detectors FL2-5). Spectral characteristics of MA allow simultaneous surface immunophenotyping. These characteristics allow multiple applications of the above-mentioned QBAs with significant diagnostic utility. They can be used as supravital fluorescent DNA probes both in fluorescence microscopy and flow cytometry including multiparameter analysis. |
Links | |
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GA525/08/0819, research and development project | Name: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA |
Investor: Czech Science Foundation, Plant sources of minor benzofenantridine alkaloids and study of heir interaction with DNA | |
LC06077, research and development project | Name: Centrum chemické genetiky |
Investor: Ministry of Education, Youth and Sports of the CR | |
MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations |
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