SLANINOVÁ, Iva, Jiří SLANINA and Eva TÁBORSKÁ. Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond. (Fluorescence properties of quaternary benzo[c]fenanthridine alkaloids and their use as a supravital DNA probes.Chemické). Chemické listy. Praha: Česká společnost chemická, 2008, vol. 102, No 102, p. 427-433. ISSN 0009-2770.
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Basic information
Original name Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond.
Name in Czech Fluorescenční vlastnosti kvartérních benzo[c]fenanthridinových alkaloidů a jejich využití jako supravitálních DNA sond.
Name (in English) Fluorescence properties of quaternary benzo[c]fenanthridine alkaloids and their use as a supravital DNA probes.Chemické
Authors SLANINOVÁ, Iva (203 Czech Republic, guarantor), Jiří SLANINA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic).
Edition Chemické listy, Praha, Česká společnost chemická, 2008, 0009-2770.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.593
RIV identification code RIV/00216224:14110/08:00025258
Organization unit Faculty of Medicine
UT WoS 000257129500004
Keywords in English benzo[c]phenanthridine alkaloids; macarpin; cell cycle; DNA probe
Tags Benzo[c]phenanthridine Alkaloids, cell cycle, DNA probe, macarpin
Tags International impact, Reviewed
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 22/6/2009 11:51.
Abstract
Práce popisuje možnost uplatnění qvartérních benzo(c)fenantridinových alkaloidů, především makarpinu, jako supravitálních fluorescenčních DNA sond vhodných pro fluorwscenční mikroskopii a průtokovou cytometrii.
Abstract (in English)
Quaternary benzo[c]phenanthridine alkaloids (QBAs) are natural products isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. They are intensively studied for their biological activities. Nevertheless they have also attractive fluorescence properties. Chromophores responsible for fluorescence are fused aromatic rings with electron-donor groups containing oxygen (OH; OCH3; OCH2O). Recently we have described fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. All these alkaloids immediately enter living cells and MA, CHR and SA bound DNA and showed nuclear architecture similar as common DNA dyes. Moreover, MA binds DNA stoichiometrically and can rapidly report the cellular DNA content in living cells at a resolution level adequate for cell cycle analysis. QBAs were excitable by common argon lasers (488 nm) emitting at the range 575 - 755 nm (i.e. fluorescence detectors FL2-5). Spectral characteristics of MA allow simultaneous surface immunophenotyping. These characteristics allow multiple applications of the above-mentioned QBAs with significant diagnostic utility. They can be used as supravital fluorescent DNA probes both in fluorescence microscopy and flow cytometry including multiparameter analysis.
Links
GA525/08/0819, research and development projectName: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA
Investor: Czech Science Foundation, Plant sources of minor benzofenantridine alkaloids and study of heir interaction with DNA
LC06077, research and development projectName: Centrum chemické genetiky
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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