Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres

KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ and Bohuslav RITTICH. Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2009, vol. 877, No 7, p. 599-602. ISSN 1570-0232.

Basic information

• Original name: Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
• Name in Czech: Izolace DNA bakteriofágů Staphylococcus aureus pro PCR prostřednictvím carboxyl funkcionalizovaných magnetických neporézních nosičů
• Authors: KAHÁNKOVÁ, Jana (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Daniel HORÁK (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor).
• Edition: Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2009, 1570-0232.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: [URL] [PDF]
• Impact factor: Impact factor: 2.777
• RIV identification code: RIV/00216224:14310/09:00029222
• Organization unit: Faculty of Science
• UT WoS: 000264225200003
• Keywords in English: Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR)
• Tags: Bacteriophage DNA, Magnetic microspheres, P(HEMA-co-EDMA), Polymerase chain reaction (PCR), Staphylococcus aureus
• Tags: International impact, Reviewed

Abstract

Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.

Abstract (in Czech)

Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.

Links

• GA203/05/2256, research and development project: Name: Magnetické hydrofilní polymerní mikročástice reagující na vnější stimuly pro lékařství a bioinženýrství

Investor: Czech Science Foundation, Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering

• GA310/09/0459, research and development project: Name: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations