| KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ a Bohuslav RITTICH. Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2009, roč. 877, č. 7, s. 599-602. ISSN 1570-0232. |
Další formáty:
BibTeX
LaTeX
RIS
@article{823220,
author = {Kahánková, Jana and Španová, Alena and Pantůček, Roman and Horák, Daniel and Doškař, Jiří and Rittich, Bohuslav},
article_location = {Amsterdam, The Netherlands},
article_number = {7},
keywords = {Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR)},
language = {eng},
issn = {1570-0232},
journal = {Journal of Chromatography B},
title = {Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres},
url = {http://linkinghub.elsevier.com/retrieve/pii/S1570023209000233},
volume = {877},
year = {2009}
}
TY - JOUR
ID - 823220
AU - Kahánková, Jana - Španová, Alena - Pantůček, Roman - Horák, Daniel - Doškař, Jiří - Rittich, Bohuslav
PY - 2009
TI - Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
JF - Journal of Chromatography B
VL - 877
IS - 7
SP - 599-602
EP - 599-602
PB - Elsevier
SN - 15700232
KW - Magnetic microspheres
KW - P(HEMA-co-EDMA)
KW - Staphylococcus aureus
KW - Bacteriophage DNA
KW - Polymerase chain reaction (PCR)
UR - http://linkinghub.elsevier.com/retrieve/pii/S1570023209000233
N2 - Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
ER -
KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ a Bohuslav RITTICH. Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2009, roč.~877, č.~7, s.~599-602. ISSN~1570-0232.
|
