Detailed Information on Publication Record
2009
Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
KAHÁNKOVÁ, Jana, Alena ŠPANOVÁ, Roman PANTŮČEK, Daniel HORÁK, Jiří DOŠKAŘ et. al.Basic information
Original name
Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
Name in Czech
Izolace DNA bakteriofágů Staphylococcus aureus pro PCR prostřednictvím carboxyl funkcionalizovaných magnetických neporézních nosičů
Authors
KAHÁNKOVÁ, Jana (203 Czech Republic), Alena ŠPANOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Daniel HORÁK (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic, guarantor)
Edition
Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2009, 1570-0232
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.777
RIV identification code
RIV/00216224:14310/09:00029222
Organization unit
Faculty of Science
UT WoS
000264225200003
Keywords in English
Magnetic microspheres; P(HEMA-co-EDMA); Staphylococcus aureus; Bacteriophage DNA; Polymerase chain reaction (PCR)
Tags
Tags
International impact, Reviewed
Změněno: 1/7/2009 18:38, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
In Czech
Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microL) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
Links
| GA203/05/2256, research and development project |
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| GA310/09/0459, research and development project |
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| MSM0021622415, plan (intention) |
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