Subcellular Localization and In Vivo Interactions of the
Arabidopsis thaliana Ethylene Receptor Family Members

J 2008

Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members

GREFEN, Christopher, Katrin STÄDELE, Kamil RŮŽIČKA, Petr OBRDLIK, Klaus HARTER et. al.

Basic information

Original name

Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members

Authors

GREFEN, Christopher (276 Germany), Katrin STÄDELE (276 Germany), Kamil RŮŽIČKA (203 Czech Republic), Petr OBRDLIK (276 Germany), Klaus HARTER (276 Germany) and Jakub HORÁK (203 Czech Republic, guarantor)

Edition

Molecular Plant, Oxford, Oxford University Press, 2008, 1674-2052

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/08:00035259

Organization unit

Faculty of Science

UT WoS

000259103700011

Keywords in English

ethylene; ethylene receptors; signal transduction; oligomerization; protein-protein interaction; MeRA; mbSUS; Arabidopsis

Abstract

V originále

The gaseous phytohormone ethylene regulates many developmental processes and responses to environmental conditions in higher plants. In Arabidopsis thaliana, ethylene perception and initiation of signaling are mediated by a family of five receptors which are related to prokaryotic two-component sensor histidine kinases. The transient expression of fluorescence-tagged receptors demonstrated that all ethylene receptors are targeted to the ER endomembrane network and do not localize to the plasmalemma. In support of in planta overlay studies, the ethylene receptors form homomeric and heteromeric protein complexes at the ER in living plant cells, as shown by membrane recruitment assays. A comparable in vivo interaction pattern was found in the yeast mating-based split-ubiquitin system. The overlapping but distinct expression pattern of the ethylene receptor genes suggests a differential composition of the ethylene receptor complexes in different plant tissues.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

GREFEN, Christopher, Katrin STÄDELE, Kamil RŮŽIČKA, Petr OBRDLIK, Klaus HARTER and Jakub HORÁK. Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members. Molecular Plant. Oxford: Oxford University Press, 2008, vol. 1, No 2, p. 308-320. ISSN 1674-2052.

@article{825598,
   author = {Grefen, Christopher and Städele, Katrin and Růžička, Kamil and Obrdlik, Petr and Harter, Klaus and Horák, Jakub},
   article_location = {Oxford},
   article_number = {2},
   keywords = {ethylene; ethylene receptors; signal transduction; oligomerization; protein-protein interaction; MeRA; mbSUS; Arabidopsis},
   language = {eng},
   issn = {1674-2052},
   journal = {Molecular Plant},
   title = {Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members},
   url = {http://mplant.oxfordjournals.org/cgi/content/full/1/2/308},
   volume = {1},
   year = {2008}
}

TY  - JOUR
ID  - 825598
AU  - Grefen, Christopher - Städele, Katrin - Růžička, Kamil - Obrdlik, Petr - Harter, Klaus - Horák, Jakub
PY  - 2008
TI  - Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members
JF  - Molecular Plant
VL  - 1
IS  - 2
SP  - 308-320
EP  - 308-320
PB  - Oxford University Press
SN  - 16742052
KW  - ethylene
KW  - ethylene receptors
KW  - signal transduction
KW  - oligomerization
KW  - protein-protein interaction
KW  - MeRA
KW  - mbSUS
KW  - Arabidopsis
UR  - http://mplant.oxfordjournals.org/cgi/content/full/1/2/308
N2  - The gaseous phytohormone ethylene regulates many developmental processes and responses to environmental conditions in higher plants. In Arabidopsis thaliana, ethylene perception and initiation of signaling are mediated by a family of five receptors which are related to prokaryotic two-component sensor histidine kinases. The transient expression of fluorescence-tagged receptors demonstrated that all ethylene receptors are targeted to the ER endomembrane network and do not localize to the plasmalemma. In support of in planta overlay studies, the ethylene receptors form homomeric and heteromeric protein complexes at the ER in living plant cells, as shown by membrane recruitment assays. A comparable in vivo interaction pattern was found in the yeast mating-based split-ubiquitin system. The overlapping but distinct expression pattern of the ethylene receptor genes suggests a differential composition of the ethylene receptor complexes in different plant tissues.
ER  -

GREFEN, Christopher, Katrin STÄDELE, Kamil RŮŽIČKA, Petr OBRDLIK, Klaus HARTER and Jakub HORÁK. Subcellular Localization and In Vivo Interactions of the Arabidopsis thaliana Ethylene Receptor Family Members. \textit{Molecular Plant}. Oxford: Oxford University Press, 2008, vol.~1, No~2, p.~308-320. ISSN~1674-2052.

Detailed Information on Publication Record