Detailed Information on Publication Record
2009
ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin
MRAVEC, Jozef, Petr SKŮPA, Aurélien BAILLY, Pavel KŘEČEK, Klára HOYEROVÁ et. al.Basic information
Original name
ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin
Name in Czech
Bunecna homeostaza fytohormonu auxinu je regulovana PIN5 transporterem v ER
Authors
MRAVEC, Jozef (703 Slovakia), Petr SKŮPA (203 Czech Republic), Aurélien BAILLY (756 Switzerland), Pavel KŘEČEK (203 Czech Republic), Klára HOYEROVÁ (203 Czech Republic), Agnieszka BIELACH (616 Poland), Jan PETRÁŠEK (203 Czech Republic), Jing ZHANG (156 China), Vassilena GAYKOVA (100 Bulgaria), York-Dieter STIERHOF (276 Germany), Kateřina SCHWARZEROVÁ (203 Czech Republic), Jakub ROLČÍK (203 Czech Republic), Petr DOBREV (804 Ukraine), Daniela SEIFERTOVÁ (203 Czech Republic), Christian LUSCHNIG (40 Austria), Eva BENKOVÁ (703 Slovakia), Eva ZAŽÍMALOVÁ (203 Czech Republic), Markus GEISLER (756 Switzerland) and Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution)
Edition
Nature, Lodon, UK, Nature Publishing Group, 2009, 0028-0836
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 34.480
RIV identification code
RIV/00216224:14310/09:00028472
Organization unit
Faculty of Science
UT WoS
000267636700045
Keywords in English
Auxin transport; auxin homeostasis; PIN carriers; endoplasmatic reticulum
Tags
International impact, Reviewed
Změněno: 20/1/2011 07:49, Mgr. Gabriela Jalová
V originále
The plant signaling molecule auxin provides positional and directional information in a wide variety of developmental processes via its differential distribution (gradients) within plant tissues1. Thus cellular auxin levels often decide about the developmental output of auxin signaling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell2. In particular, PIN auxin efflux carrier-mediated, directional auxin transport between cells is crucial for generating intercellular auxin distribution3. Here we show that Arabidopsis thaliana PIN5, - an atypical member of the PIN gene family encodes a functional auxin transporter that is required for auxin-mediated development but does not play a direct role in cell-to-cell transport. Instead, PIN5 is an important factor in controlling intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmatic reticulum (ER) presumably mediating auxin flow from the cytosol to the lumen of ER. The ER localization of other PIN5-like transporters including the moss PIN suggests that the diversification of PIN protein functions in mediating auxin homeostasis and cell-to-cell auxin transport represents an ancient event during the evolution of land plants. Sequence manipulation revealed that PIN functional diversification could have been realized by a small sequence modification in conserved residues leading to a change in subcellular protein sorting. Our data identified an additional mode of regulating homeostasis of a plant hormone by its subcellular compartmentalization and provide insights into evolution of mechanisms controlling auxin homeostasis and transport.
In Czech
Fytohormon auxin slouzi jako pozicni signal v regulaci mnoha vyvojovych procesu. Hladiny auxinu v bunce jsou regulovany auxinovym metabolismem a auxinovym transportem pres membranu, ktery zavisi na PIN auxinovych exporterech. Zde prezentujeme charakterizaci PIN5 auxinoveho transporteru, ktery nefunguje v mezibunecnem transportu auxinu, nybrz reguluje homeostazu a auxinovy metabolismus. PIN5 lokalizuje do endoplazmatickeho retikula stejne jako PIN proteiny z mechu.
Links
| IAA601630703, research and development project |
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| LC06034, research and development project |
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