| MRAVEC, Jozef, Petr SKŮPA, Aurélien BAILLY, Pavel KŘEČEK, Klára HOYEROVÁ, Agnieszka BIELACH, Jan PETRÁŠEK, Jing ZHANG, Vassilena GAYKOVA, York-Dieter STIERHOF, Kateřina SCHWARZEROVÁ, Jakub ROLČÍK, Petr DOBREV, Daniela SEIFERTOVÁ, Christian LUSCHNIG, Eva BENKOVÁ, Eva ZAŽÍMALOVÁ, Markus GEISLER and Jiří FRIML. ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin. Nature. Lodon, UK: Nature Publishing Group, 2009, Neuveden, Neuveden, 23 pp. ISSN 0028-0836. |
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@article{825698,
author = {Mravec, Jozef and Skůpa, Petr and Bailly, Aurélien and Křeček, Pavel and Hoyerová, Klára and Bielach, Agnieszka and Petrášek, Jan and Zhang, Jing and Gaykova, Vassilena and Stierhof, YorkandDieter and Schwarzerová, Kateřina and Rolčík, Jakub and Dobrev, Petr and Seifertová, Daniela and Luschnig, Christian and Benková, Eva and Zažímalová, Eva and Geisler, Markus and Friml, Jiří},
article_location = {Lodon, UK},
article_number = {Neuveden},
keywords = {Auxin transport; auxin homeostasis; PIN carriers; endoplasmatic reticulum},
language = {eng},
issn = {0028-0836},
journal = {Nature},
title = {ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin},
volume = {Neuveden},
year = {2009}
}
TY - JOUR
ID - 825698
AU - Mravec, Jozef - Skůpa, Petr - Bailly, Aurélien - Křeček, Pavel - Hoyerová, Klára - Bielach, Agnieszka - Petrášek, Jan - Zhang, Jing - Gaykova, Vassilena - Stierhof, York-Dieter - Schwarzerová, Kateřina - Rolčík, Jakub - Dobrev, Petr - Seifertová, Daniela - Luschnig, Christian - Benková, Eva - Zažímalová, Eva - Geisler, Markus - Friml, Jiří
PY - 2009
TI - ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin
JF - Nature
VL - Neuveden
IS - Neuveden
PB - Nature Publishing Group
SN - 00280836
KW - Auxin transport
KW - auxin homeostasis
KW - PIN carriers
KW - endoplasmatic reticulum
N2 - The plant signaling molecule auxin provides positional and directional information in a wide variety of developmental processes via its differential distribution (gradients) within plant tissues1. Thus cellular auxin levels often decide about the developmental output of auxin signaling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell2. In particular, PIN auxin efflux carrier-mediated, directional auxin transport between cells is crucial for generating intercellular auxin distribution3. Here we show that Arabidopsis thaliana PIN5, - an atypical member of the PIN gene family encodes a functional auxin transporter that is required for auxin-mediated development but does not play a direct role in cell-to-cell transport. Instead, PIN5 is an important factor in controlling intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmatic reticulum (ER) presumably mediating auxin flow from the cytosol to the lumen of ER. The ER localization of other PIN5-like transporters including the moss PIN suggests that the diversification of PIN protein functions in mediating auxin homeostasis and cell-to-cell auxin transport represents an ancient event during the evolution of land plants. Sequence manipulation revealed that PIN functional diversification could have been realized by a small sequence modification in conserved residues leading to a change in subcellular protein sorting. Our data identified an additional mode of regulating homeostasis of a plant hormone by its subcellular compartmentalization and provide insights into evolution of mechanisms controlling auxin homeostasis and transport.
ER -
MRAVEC, Jozef, Petr SKŮPA, Aurélien BAILLY, Pavel KŘEČEK, Klára HOYEROVÁ, Agnieszka BIELACH, Jan PETRÁŠEK, Jing ZHANG, Vassilena GAYKOVA, York-Dieter STIERHOF, Kateřina SCHWARZEROVÁ, Jakub ROLČÍK, Petr DOBREV, Daniela SEIFERTOVÁ, Christian LUSCHNIG, Eva BENKOVÁ, Eva ZAŽÍMALOVÁ, Markus GEISLER and Jiří FRIML. ER-localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin. \textit{Nature}. Lodon, UK: Nature Publishing Group, 2009, Neuveden, Neuveden, 23 pp. ISSN~0028-0836.
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