a 2009

The Nature Of Aberrant Chromosomes In Apomictic Boechera Accessions

DE JONG, Hans, Christiane KIEFER, Eric SCHRANZ, Terezie MANDÁKOVÁ, Martin LYSÁK et. al.

Basic information

Original name

The Nature Of Aberrant Chromosomes In Apomictic Boechera Accessions

Name in Czech

Původ aberantních chromosomů u apomictického rodu Boechera

Authors

DE JONG, Hans (528 Netherlands), Christiane KIEFER (40 Austria), Eric SCHRANZ (840 United States of America), Terezie MANDÁKOVÁ (203 Czech Republic), Martin LYSÁK (203 Czech Republic, guarantor) and Marcus KOCH (40 Austria)

Edition

Plant & Animal Genomes XVII Conference, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00028473

Organization unit

Faculty of Science

Keywords (in Czech)

Apomixe; chromosomová evoluce; genomové malování

Keywords in English

Apomixis; chromosome evolution; genome painting; heterochromatic chromosome
Změněno: 9/4/2010 14:06, prof. RNDr. Jiří Fajkus, CSc.

Abstract

V originále

The only apomicts in the Brassicaceae family reported so far were found in the genus Boechera and occur at the diploid, triploid and tetraploid level. Among the diploids cytotypes with 14 and 15 chromosome complements were found showing a plethora of different forms in chromosome morphology. Chromosome painting with genomic DNA of sexual B. stricta and B. holboellii (or related species) suggests that homoeologous chromosome substitution is common in these apomicts producing accessions with different chromosomes from their putative sexual ancestors. All apomicts studies so far display one or two aberrant chromosomes: a highly heterochromatic chromosome (Het) in the 14 and 15 chromosome accessions and a strikingly small chromosome (Del) in the 15 chromosome accessions. Here we report the analysis of chromosome painting experiments using pooled BACs from gene blocks defined in the sexual B. stricta. Our data showed that the Het chromosome is related to linkage group 1 (LG 1) of B. stricta and that the Del chromosome is a translocation chromosome containing the distal end of the long arm of the Het chromosome. We also confirmed a previously demonstrated translocation in BH224 and trisomy for LG7 in one apomictic 15 chromosome cytotype. The consequences of our chromosome painting for understanding related reports are discussed.

In Czech

Jediným dosud popsaným apomiktickým rodem v čeledi Brassicaceae je rod Boechera, která zahrnuje diploidní, triploidní a tetraploidní cytotypy. U diploidního cytotypu byly nalezeny dva aberantní chromosomy - vysoce heterochromatinizovaný chromosom (Het) u rostlin se čtrnácti chromosomy a malý chromosom (Del) u rostlin s patnácti chromosomy. Původ těchto aberantních chromosomů byl studován pomocí metody chromosomálního paintingu.

Links

IAA601630902, research and development project
Name: Evoluce chromosomů brukvovitých (Brassicaceae) analyzována pomocí komparativního chromosomálního paintingu
Investor: Academy of Sciences of the Czech Republic, Chromosome evolution in crucifers (Brassicaceae) revealed by comparative chromosome painting
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations