Detailed Information on Publication Record
2009
Lactobacillus spp. associated with early childhood caries
ŠVEC, Pavel, Ivo SEDLÁČEK, Lenka ŽÁČKOVÁ, Dana NOVÁKOVÁ, Martina KUKLETOVÁ et. al.Basic information
Original name
Lactobacillus spp. associated with early childhood caries
Name in Czech
Lactobacillus spp. izolované se zubního kazu v dočasné dentici
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor), Ivo SEDLÁČEK (203 Czech Republic), Lenka ŽÁČKOVÁ (203 Czech Republic), Dana NOVÁKOVÁ (203 Czech Republic) and Martina KUKLETOVÁ (203 Czech Republic)
Edition
Folia Microbiologica, 2009, 0015-5632
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.978
RIV identification code
RIV/00216224:14310/09:00035303
Organization unit
Faculty of Science
UT WoS
000265832800008
Keywords in English
Lactobacillus; early childhood caries; identification; taxonomy
Tags
International impact, Reviewed
Změněno: 18/6/2009 12:08, doc. RNDr. Pavel Švec, Ph.D.
V originále
A group of 69 lactobacilli was isolated from caries lesions and root canals of early childhood caries (ECC) affected children treated in the Department of Pedodontics (Children's Teaching Hospital, Brno, Czech Republic). Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. The rep-PCR fingerprinting with the (GTG)5 primer was used for genotypic grouping of the isolates. The (GTG)5-PCR fingerprinting grouped all analyzed strains into a few clusters in nearly full agreement with phenotype dentification results and clarified the taxonomic position of 13 biochemically unidentified strains. In total, 20 strains of Lactobacillus fermentum, 17 L. rhamnosus, 14 L. casei/paracasei, 7 L. gasseri, 7 L. salivarius and 4 L. plantarum were identified. Mixtures of two or even three Lactobacillus spp. were isolated from a few root canal content samples. Results obtained by biotyping and (GTG)5-PCR were generally comparable except for L. gasseri strains that were not biochemically identified. The (GTG)5-PCR fingerprinting was shown to be quicker, easier to perform and more reliable than biotyping. Our results imply this molecular method as a good tool for screening and identification of Lactobacillus spp. inhabiting dental plaque.
In Czech
Prace popisuje výsledek identifikace 69 kmenů laktobacilů izolovaných ze zubního kazu v dočasné dentici s vyuzitím metody (GTG)5-PCR fingerprinting.
Links
| MSM0021622416, plan (intention) |
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| 1M0528, research and development project |
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