BLÁHOVÁ, Lucie, Michal ORAVEC, Blahoslav MARŠÁLEK, Lenka ŠEJNOHOVÁ, Zdeněk ŠIMEK and Luděk BLÁHA. The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods. Toxicon. 2009, vol. 53, No 5, p. 519-524. ISSN 0041-0101.
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Basic information
Original name The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
Name in Czech První report o alkaloidním cylindrospermopsinu v ČR, analyza pomocí imunochemických metod a HPLC-MS.
Authors BLÁHOVÁ, Lucie (203 Czech Republic), Michal ORAVEC (203 Czech Republic), Blahoslav MARŠÁLEK (203 Czech Republic), Lenka ŠEJNOHOVÁ (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic, guarantor).
Edition Toxicon, 2009, 0041-0101.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30304 Public and environmental health
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.128
RIV identification code RIV/00216224:14310/09:00029252
Organization unit Faculty of Science
UT WoS 000264977700007
Keywords (in Czech) cyanotoxin;cylindrospermopsin;detekce
Keywords in English cyanotoxin;cylindrospermopsin;methods of detection
Tags cyanotoxin, cylindrospermopsin, methods of detection
Changed by Changed by: doc. RNDr. Zdeněk Šimek, CSc., učo 37019. Changed: 29/6/2009 12:55.
Abstract
We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.
Abstract (in Czech)
První celoplošná analýza obsahu cyanobakteriálního toxinu cylindrospermopsinu v povrchových vodách v České republice. Cylindrospermopsin analyzován ve vodné matrici imunochemickou (ELISA) a instrumentální metodou (LC-MS). Obě metody potvrdily jeho přítomnost ve třech lokalitách v ČR.
Links
GA524/08/0496, research and development projectName: Mechanismy nádorové promoce metabolitů toxických sinic
Investor: Czech Science Foundation, Tumor promotional mechanisms of cyanobacterial metabolites
MSM0021622412, plan (intention)Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
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