2009
The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
BLÁHOVÁ, Lucie; Michal ORAVEC; Blahoslav MARŠÁLEK; Lenka ŠEJNOHOVÁ; Zdeněk ŠIMEK et. al.Basic information
Original name
The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods
Name in Czech
První report o alkaloidním cylindrospermopsinu v ČR, analyza pomocí imunochemických metod a HPLC-MS.
Authors
BLÁHOVÁ, Lucie (203 Czech Republic); Michal ORAVEC (203 Czech Republic); Blahoslav MARŠÁLEK (203 Czech Republic); Lenka ŠEJNOHOVÁ (203 Czech Republic); Zdeněk ŠIMEK (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic, guarantor)
Edition
Toxicon, 2009, 0041-0101
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30304 Public and environmental health
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 2.128
RIV identification code
RIV/00216224:14310/09:00029252
Organization unit
Faculty of Science
UT WoS
000264977700007
Keywords (in Czech)
cyanotoxin;cylindrospermopsin;detekce
Keywords in English
cyanotoxin;cylindrospermopsin;methods of detection
Changed: 29/6/2009 12:55, doc. RNDr. Zdeněk Šimek, CSc.
In the original language
We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.
In Czech
První celoplošná analýza obsahu cyanobakteriálního toxinu cylindrospermopsinu v povrchových vodách v České republice. Cylindrospermopsin analyzován ve vodné matrici imunochemickou (ELISA) a instrumentální metodou (LC-MS). Obě metody potvrdily jeho přítomnost ve třech lokalitách v ČR.
Links
| GA524/08/0496, research and development project |
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| MSM0021622412, plan (intention) |
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