J 2009

The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

BLÁHOVÁ, Lucie, Michal ORAVEC, Blahoslav MARŠÁLEK, Lenka ŠEJNOHOVÁ, Zdeněk ŠIMEK et. al.

Basic information

Original name

The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

Name in Czech

První report o alkaloidním cylindrospermopsinu v ČR, analyza pomocí imunochemických metod a HPLC-MS.

Authors

BLÁHOVÁ, Lucie (203 Czech Republic), Michal ORAVEC (203 Czech Republic), Blahoslav MARŠÁLEK (203 Czech Republic), Lenka ŠEJNOHOVÁ (203 Czech Republic), Zdeněk ŠIMEK (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic, guarantor)

Edition

Toxicon, 2009, 0041-0101

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.128

RIV identification code

RIV/00216224:14310/09:00029252

Organization unit

Faculty of Science

UT WoS

000264977700007

Keywords (in Czech)

cyanotoxin;cylindrospermopsin;detekce

Keywords in English

cyanotoxin;cylindrospermopsin;methods of detection
Změněno: 29/6/2009 12:55, doc. RNDr. Zdeněk Šimek, CSc.

Abstract

V originále

We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.

In Czech

První celoplošná analýza obsahu cyanobakteriálního toxinu cylindrospermopsinu v povrchových vodách v České republice. Cylindrospermopsin analyzován ve vodné matrici imunochemickou (ELISA) a instrumentální metodou (LC-MS). Obě metody potvrdily jeho přítomnost ve třech lokalitách v ČR.

Links

GA524/08/0496, research and development project
Name: Mechanismy nádorové promoce metabolitů toxických sinic
Investor: Czech Science Foundation, Tumor promotional mechanisms of cyanobacterial metabolites
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)