ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK. Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates. In Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2008. p. 495. ISSN 1198-743X.
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Basic information
Original name Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.
Name in Czech Sekvenace a molekulární typizace hypervariabilních lokusů v genomech Treponema pallidum:charakterizace kmenů a klinických izolátů.
Authors ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK.
Edition Neuveden, Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704, p. 495-495, 2008.
Publisher European Society of Clinical Microbiology and Infectious Diseases
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Spain
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.554
Organization unit Faculty of Medicine
ISSN 1198-743X
Keywords (in Czech) Treponema pallidum; molekulární typizace
Keywords in English Treponema pallidum; molecular typing
Tags molecular typing, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 8/4/2010 14:22.
Abstract
Using Comparative Genomic Sequencing (CGS) approach, hypervariable regions in Treponema pallidum genome were identified. These regions comprised hypothetical genes TP0136 and TP0548. Nucleotide sequences of these genes were determined and compared in 4 groups of T. pallidum strains: in ssp. pallidum (6 strains causing syphilis), in ssp. pertenue (6 strains causing yaws), in ssp. endemicum (2 strains causing endemic syphilis) and in 1 strain of unclassified simian isolate. In addition, 9 PCR positive clinical samples were analyzed.The TP0136 and TP0548 analyzed loci showed discrete clusters in 4 groups of type strains. Analysis of strains belonging to ssp. pallidum revealed two discrete subclusters containing Nichols-like and SS14-like sequences, respectively. All clinical isolates clustered with the SS14-like subgroup.Our results showed that TP0136 and TP0548 loci represent promising targets for molecular typing of pathogenic treponemes including clinical samples.
Abstract (in Czech)
S použitím techniky CGS byly sekvencovány úseky chromosomu Treponema pallidum odpovídající genům TP0136 a TP0548. Analýza sekvence ukázala diskrétní clustery odpovídající vyšetřeným poddruhům.
Links
GA310/07/0321, research and development projectName: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum
Investor: Czech Science Foundation, Standard Projects
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
NR8967, research and development projectName: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů
Investor: Ministry of Health of the CR, Ministry of Agriculture R&D Programme
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