| ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK. Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates. In Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2008, p. 495. ISSN 1198-743X. |
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@inproceedings{829839,
author = {Šmajs, David and Matějková, Petra and Strouhal, Michal and Woznicová, Vladana and Weinstock, George M.},
address = {Neuveden},
booktitle = {Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704},
keywords = {Treponema pallidum; molecular typing},
language = {eng},
location = {Neuveden},
pages = {495-495},
publisher = {European Society of Clinical Microbiology and Infectious Diseases},
title = {Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.},
year = {2008}
}
TY - JOUR
ID - 829839
AU - Šmajs, David - Matějková, Petra - Strouhal, Michal - Woznicová, Vladana - Weinstock, George M.
PY - 2008
TI - Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.
PB - European Society of Clinical Microbiology and Infectious Diseases
CY - Neuveden
KW - Treponema pallidum
KW - molecular typing
N2 - Using Comparative Genomic Sequencing (CGS) approach, hypervariable regions in Treponema pallidum genome were identified. These regions comprised hypothetical genes TP0136 and TP0548. Nucleotide sequences of these genes were determined and compared in 4 groups of T. pallidum strains: in ssp. pallidum (6 strains causing syphilis), in ssp. pertenue (6 strains causing yaws), in ssp. endemicum (2 strains causing endemic syphilis) and in 1 strain of unclassified simian isolate. In addition, 9 PCR positive clinical samples were analyzed.The TP0136 and TP0548 analyzed loci showed discrete clusters in 4 groups of type strains. Analysis of strains belonging to ssp. pallidum revealed two discrete subclusters containing Nichols-like and SS14-like sequences, respectively. All clinical isolates clustered with the SS14-like subgroup.Our results showed that TP0136 and TP0548 loci represent promising targets for molecular typing of pathogenic treponemes including clinical samples.
ER -
ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK. Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates. In \textit{Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704}. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2008, p.~495. ISSN~1198-743X.
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