Sequencing and molecular typing of hypervariable loci in the
Treponema pallidum genomes: characterizations of type strains
and clinical isolates.

D 2008

Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.

ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ, George M. WEINSTOCK et. al.

Basic information

Original name

Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.

Name in Czech

Sekvenace a molekulární typizace hypervariabilních lokusů v genomech Treponema pallidum:charakterizace kmenů a klinických izolátů.

Authors

ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK

Edition

Neuveden, Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704, p. 495-495, 2008

Publisher

European Society of Clinical Microbiology and Infectious Diseases

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.554

Organization unit

Faculty of Medicine

ISSN

Keywords (in Czech)

Treponema pallidum; molekulární typizace

Keywords in English

Treponema pallidum; molecular typing

Abstract

ORIG CZ

V originále

Using Comparative Genomic Sequencing (CGS) approach, hypervariable regions in Treponema pallidum genome were identified. These regions comprised hypothetical genes TP0136 and TP0548. Nucleotide sequences of these genes were determined and compared in 4 groups of T. pallidum strains: in ssp. pallidum (6 strains causing syphilis), in ssp. pertenue (6 strains causing yaws), in ssp. endemicum (2 strains causing endemic syphilis) and in 1 strain of unclassified simian isolate. In addition, 9 PCR positive clinical samples were analyzed.The TP0136 and TP0548 analyzed loci showed discrete clusters in 4 groups of type strains. Analysis of strains belonging to ssp. pallidum revealed two discrete subclusters containing Nichols-like and SS14-like sequences, respectively. All clinical isolates clustered with the SS14-like subgroup.Our results showed that TP0136 and TP0548 loci represent promising targets for molecular typing of pathogenic treponemes including clinical samples.

In Czech

S použitím techniky CGS byly sekvencovány úseky chromosomu Treponema pallidum odpovídající genům TP0136 a TP0548. Analýza sekvence ukázala diskrétní clustery odpovídající vyšetřeným poddruhům.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NR8967, research and development project

Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministry of Health of the CR

Citovat

ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK. Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates. In Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2008, p. 495. ISSN 1198-743X.

@inproceedings{829839,
   author = {Šmajs, David and Matějková, Petra and Strouhal, Michal and Woznicová, Vladana and Weinstock, George M.},
   address = {Neuveden},
   booktitle = {Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704},
   keywords = {Treponema pallidum; molecular typing},
   language = {eng},
   location = {Neuveden},
   pages = {495-495},
   publisher = {European Society of Clinical Microbiology and Infectious Diseases},
   title = {Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.},
   year = {2008}
}

TY  - JOUR
ID  - 829839
AU  - Šmajs, David - Matějková, Petra - Strouhal, Michal - Woznicová, Vladana - Weinstock, George M.
PY  - 2008
TI  - Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.
PB  - European Society of Clinical Microbiology and Infectious Diseases
CY  - Neuveden
KW  - Treponema pallidum
KW  - molecular typing
N2  - Using Comparative Genomic Sequencing (CGS) approach, hypervariable regions in Treponema pallidum genome were identified. These regions comprised hypothetical genes TP0136 and TP0548. Nucleotide sequences of these genes were determined and compared in 4 groups of T. pallidum strains: in ssp. pallidum (6 strains causing syphilis), in ssp. pertenue (6 strains causing yaws), in ssp. endemicum (2 strains causing endemic syphilis) and in 1 strain of unclassified simian isolate. In addition, 9 PCR positive clinical samples were analyzed.The TP0136 and TP0548 analyzed loci showed discrete clusters in 4 groups of type strains. Analysis of strains belonging to ssp. pallidum revealed two discrete subclusters containing Nichols-like and SS14-like sequences, respectively. All clinical isolates clustered with the SS14-like subgroup.Our results showed that TP0136 and TP0548 loci represent promising targets for molecular typing of pathogenic treponemes including clinical samples.
ER  -

ŠMAJS, David, Petra MATĚJKOVÁ, Michal STROUHAL, Vladana WOZNICOVÁ and George M. WEINSTOCK. Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates. In \textit{Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704}. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2008, p.~495. ISSN~1198-743X.

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