Detailed Information on Publication Record
2009
Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans
MUSILOVÁ, Jindra, Vojtěch SEDLÁČEK, Igor KUČERA and Zdeněk GLATZBasic information
Original name
Capillary zone electrophoresis with field enhanced sample stacking as a tool for targeted metabolome analysis of adenine nucleotides and coenzymes in Paracoccus denitrificans
Name in Czech
Využití kombinace CZE a stackingu pro metabolomickou analýzu adeninových nucleotidů a koenzymů v bakterii Paracoccus denitrificans
Authors
MUSILOVÁ, Jindra (203 Czech Republic, belonging to the institution), Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution), Igor KUČERA (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2009, 1615-9306
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.551
RIV identification code
RIV/00216224:14310/09:00035508
Organization unit
Faculty of Science
UT WoS
000268595800011
Keywords in English
Adenine Nucleotides and Coenzymes; Capillary zone electrophoresis; Field enhanced sample stacking; Metabolomics; Paracoccus denitrificans
Tags
Tags
International impact, Reviewed
Změněno: 27/6/2012 16:19, prof. RNDr. Zdeněk Glatz, CSc.
V originále
The main aim of this work was to demonstrate the applicability of capillary zone electrophoresis in combination with field enhanced sample stacking in targeted metabolome analyses of adenine nucleotides - AMP, ADP, ATP, coenzymes NAD+, NADP+ and their reduced forms in Paracoccus denitrificans. 100 mM sodium carbonate/hydrogencarbonate buffer (pH 9.6) with the addition of beta-cyclodextrin at a concentration of 10 mM was found to be an effective background electrolyte for their separation within 20 minutes. Besides this, special attention was paid to the development of the procedure for the extraction of specific metabolites from the bacterium Paracoccus denitrificans. This procedure was not only optimised to achieve the highest metabolite yields but also to obtain a sample that was fully compatible with the on-line preconcetration strategy used. The developed methodology was finally applied in a study of the bacterium P. denitrificans at various stages of the active respiratory chain.
In Czech
Bylo využito kombinace CZE a stackingu pro metabolomickou analýzu adeninových nucleotidů a koenzymů v bakterii Paracoccus denitrificans.
Links
| LC06023, research and development project |
| ||
| MSM0021622413, plan (intention) |
|