This paper addresses climatic fluctuation in the Czech Republic in the period 1961-2005. The fluctuations in monthly, seasonal and annual series of selected climatological characteristics are analysed. The annual series of mean, maximum and minimum air temperature, daily temperature range and sunshine duration exhibit a rising linear trend, in contrast to dropping trends in relative air humidity, number of days with snow cover and mean wind speed. There are no pronounced changes in precipitation totals, although their distribution over the course of the year becomes more regular. Temperature trends, with the exception of autumn, show a clear enhancement since the 1980s. Linkage to fluctuation was studied using NAOI and CEZI indices as indicators of circulation patterns in Central Europe.