DNA repair mechanisms in yeast

KREJČÍ, Lumír. DNA repair mechanisms in yeast. In 37th Annual Conference on Yeast. 37th Annual Conference o. Bratislava, Slovakia: Visegrad Strategic Program. 113 s. ISSN 1336-4839. 2009.

Základní údaje

• Originální název: DNA repair mechanisms in yeast
• Název česky: DNA opravné mechanismy u kvasinek
• Autoři: KREJČÍ, Lumír.
• Vydání: 37th Annual Conference o. Bratislava, Slovakia, 37th Annual Conference on Yeast, 113 s. 2009.
• Nakladatel: Visegrad Strategic Program

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Stať ve sborníku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Slovensko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Organizační jednotka: Lékařská fakulta
• ISSN: 1336-4839
• Klíčová slova anglicky: DNA repair; DNA damage; replication; genomic instability
• Štítky: DNA damage, DNA repair, genomic instability, replication
• Příznaky: Mezinárodní význam

Anotace

DNA replication is typically highly processive mechanism with astonishing precision, despite the fact that it frequently encounters barriers caused by endogenous and exogenous genotoxic agents. It is not surprising that DNA replication does not act alone, but operates in coordination with homologous recombination (HR), and other DNA repair processes to overcome such replication obstacles to ensure cellular viability, and achieve genomic stability. Numerous mechanisms by which replication forks can be restarted following arrest have been described. Some of the pathways enable the cells to deal with such impediments while keeping the fork in place, including repriming, template switch, and translesion synthesis, others may provoke partial or complete fork collapse followed by fork reversal. This could not only provide time and space for repair but also liberate the new strand to undergo template switch. Alternatively, the fork can be cleaved, creating the DSB break that is repaired by recombination machinery with ability to restart the replication fork. Inability to remove toxic DNA structures often leads to their accumulation, higher mutation frequency and eventually to cancer or other diseases associated with genomic instability.

Anotace česky

DNA replication is typically highly processive mechanism with astonishing precision, despite the fact that it frequently encounters barriers caused by endogenous and exogenous genotoxic agents. It is not surprising that DNA replication does not act alone, but operates in coordination with homologous recombination (HR), and other DNA repair processes to overcome such replication obstacles to ensure cellular viability, and achieve genomic stability. Numerous mechanisms by which replication forks can be restarted following arrest have been described. Some of the pathways enable the cells to deal with such impediments while keeping the fork in place, including repriming, template switch, and translesion synthesis, others may provoke partial or complete fork collapse followed by fork reversal. This could not only provide time and space for repair but also liberate the new strand to undergo template switch. Alternatively, the fork can be cleaved, creating the DSB break that is repaired by recombination machinery with ability to restart the replication fork. Inability to remove toxic DNA structures often leads to their accumulation, higher mutation frequency and eventually to cancer or other diseases associated with genomic instability.

Návaznosti

• GA301/09/1917, projekt VaV: Název: Štěpení replikačních-rekombinačních DNA meziproduktů a jejich úloha při nestabilitě genomu

Investor: Grantová agentura ČR, Štěpení replikačních-rekombinačních DNA meziproduktů a jejich úloha při nestabilitě genomu

• GD203/09/H046, projekt VaV: Název: Biochemie na rozcestí mezi in silico a in vitro

Investor: Grantová agentura ČR, Biochemie na rozcestí mezi in silico a in vitro

• LC06030, projekt VaV: Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

• MSM0021622413, záměr: Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím