DNA repair mechanisms in yeast

KREJČÍ, Lumír. DNA repair mechanisms in yeast. In 37th Annual Conference on Yeast. 37th Annual Conference o. Bratislava, Slovakia: Visegrad Strategic Program, 2009, 113 pp. ISSN 1336-4839.

Basic information

• Original name: DNA repair mechanisms in yeast
• Name in Czech: DNA opravné mechanismy u kvasinek
• Authors: KREJČÍ, Lumír.
• Edition: 37th Annual Conference o. Bratislava, Slovakia, 37th Annual Conference on Yeast, 113 pp. 2009.
• Publisher: Visegrad Strategic Program

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Medicine
• ISSN: 1336-4839
• Keywords in English: DNA repair; DNA damage; replication; genomic instability
• Tags: DNA damage, DNA repair, genomic instability, replication
• Tags: International impact

Abstract

DNA replication is typically highly processive mechanism with astonishing precision, despite the fact that it frequently encounters barriers caused by endogenous and exogenous genotoxic agents. It is not surprising that DNA replication does not act alone, but operates in coordination with homologous recombination (HR), and other DNA repair processes to overcome such replication obstacles to ensure cellular viability, and achieve genomic stability. Numerous mechanisms by which replication forks can be restarted following arrest have been described. Some of the pathways enable the cells to deal with such impediments while keeping the fork in place, including repriming, template switch, and translesion synthesis, others may provoke partial or complete fork collapse followed by fork reversal. This could not only provide time and space for repair but also liberate the new strand to undergo template switch. Alternatively, the fork can be cleaved, creating the DSB break that is repaired by recombination machinery with ability to restart the replication fork. Inability to remove toxic DNA structures often leads to their accumulation, higher mutation frequency and eventually to cancer or other diseases associated with genomic instability.

Abstract (in Czech)

DNA replication is typically highly processive mechanism with astonishing precision, despite the fact that it frequently encounters barriers caused by endogenous and exogenous genotoxic agents. It is not surprising that DNA replication does not act alone, but operates in coordination with homologous recombination (HR), and other DNA repair processes to overcome such replication obstacles to ensure cellular viability, and achieve genomic stability. Numerous mechanisms by which replication forks can be restarted following arrest have been described. Some of the pathways enable the cells to deal with such impediments while keeping the fork in place, including repriming, template switch, and translesion synthesis, others may provoke partial or complete fork collapse followed by fork reversal. This could not only provide time and space for repair but also liberate the new strand to undergo template switch. Alternatively, the fork can be cleaved, creating the DSB break that is repaired by recombination machinery with ability to restart the replication fork. Inability to remove toxic DNA structures often leads to their accumulation, higher mutation frequency and eventually to cancer or other diseases associated with genomic instability.

Links

• GA301/09/1917, research and development project: Name: Štěpení replikačních-rekombinačních DNA meziproduktů a jejich úloha při nestabilitě genomu

Investor: Czech Science Foundation

• GD203/09/H046, research and development project: Name: Biochemie na rozcestí mezi in silico a in vitro

Investor: Czech Science Foundation

• LC06030, research and development project: Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment