| ANTONY, Edwin, Eric J. TOMKO, Qi XIAO, Lumír KREJČÍ, Timothy M. LOHMAN and Tom ELLENBERGER. Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA. Molecular Cell. 2009, vol. 35, No 1, p. 105-115, 10 pp. ISSN 1097-2765. |
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@article{831723,
author = {Antony, Edwin and Tomko, Eric J. and Xiao, Qi and Krejčí, Lumír and Lohman, Timothy M. and Ellenberger, Tom},
article_number = {1},
keywords = {DNA repair; DNA damage; replication; genomic instability},
language = {eng},
issn = {1097-2765},
journal = {Molecular Cell},
title = {Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA},
volume = {35},
year = {2009}
}
TY - JOUR
ID - 831723
AU - Antony, Edwin - Tomko, Eric J. - Xiao, Qi - Krejčí, Lumír - Lohman, Timothy M. - Ellenberger, Tom
PY - 2009
TI - Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA
JF - Molecular Cell
VL - 35
IS - 1
SP - 105-115
EP - 105-115
SN - 10972765
KW - DNA repair
KW - DNA damage
KW - replication
KW - genomic instability
N2 - Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instability and it is normally prevented by remodeling enzymes that antagonize the activities of Rad51 nucleoprotein filaments. In yeast, the Srs2 helicase/translocase suppresses HR by clearing Rad51 polymers from single-stranded DNA. We have examined the mechanism of disassembly of Rad51 nucleoprotein filaments by Srs2 and find that a physical interaction between Rad51 and the C-terminal region of Srs2 triggers ATP hydrolysis within the Rad51 filament, causing Rad51 to dissociate from DNA. This allosteric mechanism explains the biological specialization of Srs2 as a DNA motor protein that antagonizes HR.
ER -
ANTONY, Edwin, Eric J. TOMKO, Qi XIAO, Lumír KREJČÍ, Timothy M. LOHMAN and Tom ELLENBERGER. Srs2 Disassembles Rad51 Filaments by a Protein-Protein Interaction Triggering ATP Turnover and Dissociation of Rad51 from DNA. \textit{Molecular Cell}. 2009, vol.~35, No~1, p.~105-115, 10 pp. ISSN~1097-2765.
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