MALDI MS profiling of bacteria

ŠEDO, Ondrej, Aleš VORÁČ, Andrea TESHIM, Alexandr NEMEC, Ludmila TVRZOVÁ, Ivo SEDLÁČEK and Zbyněk ZDRÁHAL. MALDI MS profiling of bacteria. In 27th Informal Meeting on Mass Spectrometry. 2009. ISBN 978-3-200-01508-1.

Basic information

• Original name: MALDI MS profiling of bacteria
• Name in Czech: MALDI MS profilování bakterií
• Name (in English): MALDI MS profiling of bacteria
• Authors: ŠEDO, Ondrej (203 Czech Republic), Aleš VORÁČ (203 Czech Republic), Andrea TESHIM (203 Czech Republic), Alexandr NEMEC (203 Czech Republic), Ludmila TVRZOVÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor).
• Edition: 27th Informal Meeting on Mass Spectrometry, 2009.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/09:00035530
• Organization unit: Faculty of Science
• ISBN: 978-3-200-01508-1
• Keywords (in Czech): MALDI MS;hmotnostní spektrometrie;bakterie
• Keywords in English: MALDI MS;mass spectrometry;bacteria
• Tags: bacteria, MALDI MS, mass spectrometry

Abstract

MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.

Abstract (in English)

MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time