a 2009

MALDI MS profiling of bacteria

ŠEDO, Ondrej, Aleš VORÁČ, Andrea TESHIM, Alexandr NEMEC, Ludmila TVRZOVÁ et. al.

Basic information

Original name

MALDI MS profiling of bacteria

Name in Czech

MALDI MS profilování bakterií

Name (in English)

MALDI MS profiling of bacteria

Authors

ŠEDO, Ondrej (203 Czech Republic), Aleš VORÁČ (203 Czech Republic), Andrea TESHIM (203 Czech Republic), Alexandr NEMEC (203 Czech Republic), Ludmila TVRZOVÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor)

Edition

27th Informal Meeting on Mass Spectrometry, 2009

Other information

Language

Czech

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00035530

Organization unit

Faculty of Science

ISBN

978-3-200-01508-1

Keywords (in Czech)

MALDI MS;hmotnostní spektrometrie;bakterie

Keywords in English

MALDI MS;mass spectrometry;bacteria
Změněno: 31/3/2010 09:58, prof. RNDr. Ivo Sedláček, CSc.

Abstract

V originále

MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.

In English

MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.

Links

LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time