Diversity of prophages in dominant Staphylococcus aureus>
clonal lineages

J 2009

Diversity of prophages in dominant Staphylococcus aureus> clonal lineages

GOERKE, Christiane, Roman PANTŮČEK, Silvia HOLTFRETER, Berit SCHULTE, Manuel ZINK et. al.

Basic information

Original name

Diversity of prophages in dominant Staphylococcus aureus> clonal lineages

Name in Czech

Rozmanitost profágů u dominantních klonálních linií Staphylococcus aureus

Authors

GOERKE, Christiane (276 Germany), Roman PANTŮČEK (203 Czech Republic, guarantor), Silvia HOLTFRETER (276 Germany), Berit SCHULTE (276 Germany), Manuel ZINK (276 Germany), Dorothee GRUMANN (276 Germany), Barbara M. BRÖKER (276 Germany), Jiří DOŠKAŘ (203 Czech Republic) and Christiane WOLZ (276 Germany)

Edition

Journal of Bacteriology, Washington, D.C. American Society for Microbiology, 2009, 0021-9193

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[PDF] [URL]

Impact factor

Impact factor: 3.940

RIV identification code

RIV/00216224:14310/09:00035545

Organization unit

Faculty of Science

UT WoS

000266041300005

Keywords in English

Staphylococcus aureus; bacteriophages; accessory genome; clonal lineages; lysogeny

Abstract

ORIG CZ

V originále

Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Seventy-one published genome sequences of staphylococcal phages were clustered into distinct integrase groups which were related to the chromosomal integration site and to the encoded virulence gene content. Analysis of three marker modules (lysogeny, tail, and lysis) for phage functional units revealed that these phages exhibit different degrees of genome mosaicism. The prevalence of prophages in a representative S. aureus strain collection consisting of 386 isolates of diverse origin was determined. By linking the phage content to dominant S. aureus clonal complexes we could show that the distribution of bacteriophages varied remarkably between lineages, indicating restriction-based barriers. A comparison of colonizing and invasive S. aureus strain populations revealed that hlb-converting phages were significantly more frequent in colonizing strains.

In Czech

Temperované fágy hrají významnou úlohu v patogenitě Staphylococcus aureus, např. zprostředkovávají horizontální přenos genů virulence a rezistence. V této práci navrhujeme klasifikační schéma profágů čeledi siphoviridae na základě polymorfizmu genu pro integrázu.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

GOERKE, Christiane, Roman PANTŮČEK, Silvia HOLTFRETER, Berit SCHULTE, Manuel ZINK, Dorothee GRUMANN, Barbara M. BRÖKER, Jiří DOŠKAŘ and Christiane WOLZ. Diversity of prophages in dominant Staphylococcus aureus> clonal lineages. Journal of Bacteriology. Washington, D.C.: American Society for Microbiology, 2009, vol. 191, No 11, p. 3462-3468. ISSN 0021-9193.

@article{832297,
   author = {Goerke, Christiane and Pantůček, Roman and Holtfreter, Silvia and Schulte, Berit and Zink, Manuel and Grumann, Dorothee and Bröker, Barbara M. and Doškař, Jiří and Wolz, Christiane},
   article_location = {Washington, D.C.},
   article_number = {11},
   keywords = {Staphylococcus aureus; bacteriophages; accessory genome; clonal lineages; lysogeny},
   language = {eng},
   issn = {0021-9193},
   journal = {Journal of Bacteriology},
   title = {Diversity of prophages in dominant Staphylococcus aureus> clonal lineages},
   url = {http://jb.asm.org/cgi/content/full/191/11/3462},
   volume = {191},
   year = {2009}
}

TY  - JOUR
ID  - 832297
AU  - Goerke, Christiane - Pantůček, Roman - Holtfreter, Silvia - Schulte, Berit - Zink, Manuel - Grumann, Dorothee - Bröker, Barbara M. - Doškař, Jiří - Wolz, Christiane
PY  - 2009
TI  - Diversity of prophages in dominant Staphylococcus aureus> clonal lineages
JF  - Journal of Bacteriology
VL  - 191
IS  - 11
SP  - 3462-3468
EP  - 3462-3468
PB  - American Society for Microbiology
SN  - 00219193
KW  - Staphylococcus aureus
KW  - bacteriophages
KW  - accessory genome
KW  - clonal lineages
KW  - lysogeny
UR  - http://jb.asm.org/cgi/content/full/191/11/3462
N2  - Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating the horizontal gene transfer of virulence factors. Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. Seventy-one published genome sequences of staphylococcal phages were clustered into distinct integrase groups which were related to the chromosomal integration site and to the encoded virulence gene content. Analysis of three marker modules (lysogeny, tail, and lysis) for phage functional units revealed that these phages exhibit different degrees of genome mosaicism. The prevalence of prophages in a representative S. aureus strain collection consisting of 386 isolates of diverse origin was determined. By linking the phage content to dominant S. aureus clonal complexes we could show that the distribution of bacteriophages varied remarkably between lineages, indicating restriction-based barriers. A comparison of colonizing and invasive S. aureus strain populations revealed that hlb-converting phages were significantly more frequent in colonizing strains.
ER  -

GOERKE, Christiane, Roman PANTŮČEK, Silvia HOLTFRETER, Berit SCHULTE, Manuel ZINK, Dorothee GRUMANN, Barbara M. BRÖKER, Jiří DOŠKAŘ and Christiane WOLZ. Diversity of prophages in dominant Staphylococcus aureus$>$ clonal lineages. \textit{Journal of Bacteriology}. Washington, D.C.: American Society for Microbiology, 2009, vol.~191, No~11, p.~3462-3468. ISSN~0021-9193.

Detailed Information on Publication Record