MANDL, Martin, Romana MARKOVÁ and Antonín LOJEK. ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans. Advanced Materials Research. Switzerland: Trans Tech Publication, 2009, 71-73, No 6, p. 275-278. ISSN 1022-6680.
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Basic information
Original name ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans
Name in Czech Meření ATP v železooxidující bakterii Acidithiobacillus ferrooxidans
Authors MANDL, Martin (203 Czech Republic, guarantor, belonging to the institution), Romana MARKOVÁ (203 Czech Republic) and Antonín LOJEK (203 Czech Republic, belonging to the institution).
Edition Advanced Materials Research, Switzerland, Trans Tech Publication, 2009, 1022-6680.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 20700 2.7 Environmental engineering
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/09:00042514
Organization unit Faculty of Science
UT WoS 000273541600062
Keywords (in Czech) Acidithiobacillus ferrooxidans; ATP; bakterie oxidující železo
Keywords in English Acidithiobacillus ferrooxidans; ATP; iron-oxidizing bacteria
Tags Acidithiobacillus ferrooxidans, ATP, Iron-oxidizing bacteria
Tags International impact, Reviewed
Changed by Changed by: doc. Ing. Martin Mandl, CSc., učo 1408. Changed: 13/1/2016 09:54.
Abstract
We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1 . 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 . 1012 cells per mol Fe(II).
Abstract (in Czech)
Byla studována aplikace kitu pro stanovení ATP na bakterii Acidithiobacillus ferrooxidans osidujcící Fe(II). Byly stanoveny základní chemometrické parametry a podmínky použití na reálnou rostoucí kulturu.
Links
GA525/08/0697, research and development projectName: Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek
Investor: Czech Science Foundation, Activity of acidophilic sulfur-oxidizing bacteria under aerobic and anaerobic conditions
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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