Detailed Information on Publication Record
2009
ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans
MANDL, Martin, Romana MARKOVÁ and Antonín LOJEKBasic information
Original name
ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans
Name in Czech
Meření ATP v železooxidující bakterii Acidithiobacillus ferrooxidans
Authors
MANDL, Martin (203 Czech Republic, guarantor, belonging to the institution), Romana MARKOVÁ (203 Czech Republic) and Antonín LOJEK (203 Czech Republic, belonging to the institution)
Edition
Advanced Materials Research, Switzerland, Trans Tech Publication, 2009, 1022-6680
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20700 2.7 Environmental engineering
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/09:00042514
Organization unit
Faculty of Science
UT WoS
000273541600062
Keywords (in Czech)
Acidithiobacillus ferrooxidans; ATP; bakterie oxidující železo
Keywords in English
Acidithiobacillus ferrooxidans; ATP; iron-oxidizing bacteria
Tags
International impact, Reviewed
Změněno: 13/1/2016 09:54, doc. Ing. Martin Mandl, CSc.
V originále
We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1 . 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 . 1012 cells per mol Fe(II).
In Czech
Byla studována aplikace kitu pro stanovení ATP na bakterii Acidithiobacillus ferrooxidans osidujcící Fe(II). Byly stanoveny základní chemometrické parametry a podmínky použití na reálnou rostoucí kulturu.
Links
| GA525/08/0697, research and development project |
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| MSM0021622413, plan (intention) |
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