MATALOVÁ, Eva, Ron RADLANSKI, Ivan MÍŠEK and Jan ŠMARDA. c-Myb expression at different stages of molar tooth germ. 2009.
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Basic information
Original name c-Myb expression at different stages of molar tooth germ
Name in Czech Exprese c-Myb v různých stádiích vývoje molárů
Authors MATALOVÁ, Eva (203 Czech Republic), Ron RADLANSKI (276 Germany), Ivan MÍŠEK (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor).
Edition 2009.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/09:00035656
Organization unit Faculty of Science
UT WoS 000265436200360
Keywords (in Czech) c-myb; odontogeneze; molár
Keywords in English c-Myb; odontogenesis; molar tooth
Tags c-Myb, molar tooth, Odontogenesis
Changed by Changed by: prof. RNDr. Jan Šmarda, CSc., učo 1223. Changed: 1/4/2010 15:25.
Abstract
The aim of this work was to show c-myb expression profile in temporo-spatial model of mouse molar tooth germ development in the context of related tissues. Expression of c-myb (Abcam polyclonal antibody) was followed together with proliferation analysis (PCNAproliferating cell nuclear antigen, DAKO monoclonal antibody) and apoptosis detection (TUNEL test, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Chemicon). Secondary biotinylated antibody was labelled by streptavidin-POD to visualize positive cells by addition of DAB substrate. c-myb, PCNA and TUNEL were evaluated in the epithelial and mesenchymal parts of the tooth germ and in the surrounding interdental bone.
Abstract (in Czech)
Cílem tohoto projektu bylo zjištění časo-prostorového schématu exprese genu c-myb během vývoje myšího moláru.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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