Targeting the G-quadruplex-forming region near the P1 promoter
in the human BCL-2 gene with the cationic porphyrin TMPyP4 and
the complementary C-rich strand

J 2009

Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand

DEL TORO, Miguel, Pavel BUČEK, Anna AVIÑÓ, Joaquim JAUMOT, Carlos GONZALES et. al.

Basic information

Original name

Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand

Name in Czech

Zaměření sekvence G-kvadruplexu na promotoru P1 lidského genu BCL-2 pomocí kationického porfyrinu TMPyP4 a komplementární sekvence, bohaté na cytosin

Authors

DEL TORO, Miguel (724 Spain), Pavel BUČEK (203 Czech Republic, guarantor, belonging to the institution), Anna AVIÑÓ (724 Spain), Joaquim JAUMOT (724 Spain), Carlos GONZALES (724 Spain), Ramon ERITJA (724 Spain) and Raimundo GARGALLO (724 Spain)

Edition

Biochimie, Paris, Elsevier Masson SAS, 2009, 0300-9084

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

ScienceDirect Article DOI: 10.1016/j.biochi.2009.04.012

Impact factor

Impact factor: 3.897

RIV identification code

RIV/00216224:14310/09:00035680

Organization unit

Faculty of Science

UT WoS

000267628700011

Keywords in English

Oligonucleotides;G_quadruplex; bcl-2;TMPyP4;Spectroscopy;Multivariate analysis

Abstract

ORIG CZ

V originále

The interaction of BCL-2 G-quadruplex forming sequence with cationic porphyrin TMPyP4 and its C-rich complementary strand was studied by means of molecular absorption spectroscopy and circular dichroism, experimental dates were analysed by advanced chemometric techniques based on mulitvariate analysis. Analyses resulted in proving 1:2 stoichiometry for the complex BCL-2G:TMPyP4 and equilibrium constant 5,0*10^13 M-2, that is weaker than complex of BCL-2 complementary strands, equilibrium constant of which was determined 10^7,7 M-1.

In Czech

Studie interakcí oligonukleotidu bohatého na guanin, tvořícího G-kvadruplex, s kationickým porfyrinem TMPyP4 a komplementárním vláknem DNA. Experimenty byly monitorovány pomocí molek. absorpční spektroskopie a cirkulárního dichroismu, analýza dat pokročilými chemometrickými metodani založenými na multivariátní analýze. Výsledky potvrdily navrženou stechiometrii komplexu BCL-2G:TMPyP4 1:2, s konstantou stability 5,0*10^13 M-2. Duplex obou komplementárních sekvencí BCL-2 je silnější, konstanta stability 10^7,7 M-1.

Citovat

DEL TORO, Miguel, Pavel BUČEK, Anna AVIÑÓ, Joaquim JAUMOT, Carlos GONZALES, Ramon ERITJA and Raimundo GARGALLO. Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand. Biochimie. Paris: Elsevier Masson SAS, 2009, vol. 91, No 7, p. 894-902. ISSN 0300-9084.

@article{834828,
   author = {del Toro, Miguel and Buček, Pavel and Aviñó, Anna and Jaumot, Joaquim and Gonzales, Carlos and Eritja, Ramon and Gargallo, Raimundo},
   article_location = {Paris},
   article_number = {7},
   keywords = {Oligonucleotides;G_quadruplex; bcl-2;TMPyP4;Spectroscopy;Multivariate analysis},
   language = {eng},
   issn = {0300-9084},
   journal = {Biochimie},
   title = {Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand},
   url = {http://www.sciencedirect.com/science/article/pii/S0300908409001126},
   volume = {91},
   year = {2009}
}

TY  - JOUR
ID  - 834828
AU  - del Toro, Miguel - Buček, Pavel - Aviñó, Anna - Jaumot, Joaquim - Gonzales, Carlos - Eritja, Ramon - Gargallo, Raimundo
PY  - 2009
TI  - Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand
JF  - Biochimie
VL  - 91
IS  - 7
SP  - 894-902
EP  - 894-902
PB  - Elsevier Masson SAS
SN  - 03009084
KW  - Oligonucleotides;G_quadruplex
KW  - bcl-2;TMPyP4;Spectroscopy;Multivariate analysis
UR  - http://www.sciencedirect.com/science/article/pii/S0300908409001126
N2  - The interaction of BCL-2 G-quadruplex forming sequence with cationic porphyrin TMPyP4 and its C-rich complementary strand was studied by means of molecular absorption spectroscopy and circular dichroism, experimental dates were analysed by advanced chemometric techniques based on mulitvariate analysis. Analyses resulted in proving 1:2 stoichiometry for the complex BCL-2G:TMPyP4 and equilibrium constant 5,0*10^13 M-2, that is weaker than complex of BCL-2 complementary strands, equilibrium constant of which was determined 10^7,7 M-1.
ER  -

DEL TORO, Miguel, Pavel BUČEK, Anna AVIÑÓ, Joaquim JAUMOT, Carlos GONZALES, Ramon ERITJA and Raimundo GARGALLO. Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand. \textit{Biochimie}. Paris: Elsevier Masson SAS, 2009, vol.~91, No~7, p.~894-902. ISSN~0300-9084.

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